*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
chicken fibroblasts, human foreskin fibroblasts
The antibody recognizes the two major and one of the minor β‑tubulin isotypes of the free-living soil nematode C. elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, to neuronal axons of the larva of an ascidian (Ciona intestinalis) and to tubulin of chicken and mammals, cultured human fibroblasts, bovine and rat brain tissue. In C.elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and β-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels. It reacts with heat-denatured and reduced mammalian preparations in immunoblotting.
Ascites, 15 mM sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
P07437, OK/SWcl.56, M40, MGC16435, TUBB5, 191130, TUBB, beta Tubulin, OK/SW-cl.56, MGC117247, TUBB1, 203068, OK/SW cl.56
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.
GTX11311 WB Image
WB analysis of chicken fibroblasts extract using GTX11311 beta Tubulin antibody [2-28-33].
Lane A : Antibody dilution 1:200
Lane B : Negative control (only secondary antibody)