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beta Tubulin antibody [2-28-33]

Anti-beta Tubulin antibody [2-28-33] used in Western Blot (WB). GTX11311

Cat No. GTX11311

Host Mouse
Clonality Monoclonal
Clone Name 2-28-33
Isotype IgG1
Application WB, ICC/IF
Reactivity Human, Rat, Bovine, Chicken, Caenorhabditis elegans, Ciona intestinalis, Strongylocentrotus purpuratus
Package
200 μl ($489)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100
ICC/IF 1:1,000
Not tested in other applications.

Positive Control

chicken fibroblasts, human foreskin fibroblasts

Specificity/Sensitivity

The antibody recognizes the two major and one of the minor β‑tubulin isotypes of the free-living soil nematode C. elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, to neuronal axons of the larva of an ascidian (Ciona intestinalis) and to tubulin of chicken and mammals, cultured human fibroblasts, bovine and rat brain tissue. In C.elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and β-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels. It reacts with heat-denatured and reduced mammalian preparations in immunoblotting.
PROPERTIES

Form

Liquid

Buffer

Ascites, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Sea Urchin

Immunogen

sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P07437, OK/SWcl.56, M40, MGC16435, TUBB5, 191130, TUBB, beta Tubulin, OK/SW-cl.56, MGC117247, TUBB1, 203068, OK/SW cl.56

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Research Area

DATA IMAGES
Anti-beta Tubulin antibody [2-28-33] used in Western Blot (WB). GTX11311

GTX11311 WB Image

WB analysis of chicken fibroblasts extract using GTX11311 beta Tubulin antibody [2-28-33].
Lane A : Antibody dilution 1:200
Lane B : Negative control (only secondary antibody)

REFERENCE
There are currently no references for beta Tubulin antibody [2-28-33] (GTX11311). Be the first to share your publications with this product.
REVIEW
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