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beta Tubulin antibody [D66]

Anti-beta Tubulin antibody [D66] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11307
Anti-beta Tubulin antibody [D66] used in Western Blot (WB). GTX11307

Cat No. GTX11307

Host Mouse
Clonality Monoclonal
Clone Name D66
Isotype IgG1
Application WB, ICC/IF, IP, ELISA
Reactivity Human, Mouse, Rat, Rabbit, Bovine, Dog, Hamster, Chicken, Monkey, Chlamydomonas reinhardtii, Dinoflagellate, Sea Urchin
Package
100 μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0 μg/ml
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Positive Control

PC12

Specificity/Sensitivity

Recognizes all mouse brain β-tubulin isoforms (β and β′ subpopulations), and sea urchin β2-tubulin. The epitope recognized by the antibody is localized in the C-terminal region of β-tubulin (amino acids 427-432 in sea urchin β2-tubulin).
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 1% BSA, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~1? mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Sea Urchin

Immunogen

sea urchin (Lytechinus pictus) sperm axonemal proteins.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P07437, OK/SWcl.56, M40, MGC16435, TUBB5, 191130, TUBB, beta Tubulin, OK/SW-cl.56, MGC117247, TUBB1, 203068, OK/SW cl.56

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Research Area

DATA IMAGES
Anti-beta Tubulin antibody [D66] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11307

GTX11307 ICC/IF Image

ICC/IF analysis of SH-SY-5Y cells using GTX11307 beta Tubulin antibody [D66] at 0.5 μg/mL(red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

Anti-beta Tubulin antibody [D66] used in Western Blot (WB). GTX11307

GTX11307 WB Image

WB analysis of (1) HeLa, (2) U87, (3) P19, (4) Neuro-2a, (5) PC-12, (6) COS-7, (7) CHO, (8) MDBK and (9) MDCK lysates using beta Tubulin antibody [D66] at of 1 μg/ml.

REFERENCE
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