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beta Tubulin antibody [DM1B]

Cat No. GTX27287

Host Mouse
Clonality Monoclonal
Clone Name DM1B
Isotype IgG1
Application WB, ICC/IF
Reactivity Human, Mouse, Rat, Gerbil
Package
100 μg ($319)
APPLICATION

Application Note

For IF: Use at a concentration of 4 - 6 μg/ m l. For WB: Use at a concentration of 4 -6μg/ m l. Detects a band of approximately 60 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

Any cell line
PROPERTIES

Form

Liquid

Buffer

50 mM sodium phosphate buffer containing 0.2% gelatin and =0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

Native chicken brain microtubules

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P07437, OK/SWcl.56, M40, MGC16435, TUBB5, 191130, TUBB, beta Tubulin, OK/SW-cl.56, MGC117247, TUBB1, 203068, OK/SW cl.56

Background

Cytoskeletal proteins are found in highly organized arrays within the cytoplasm of higher eukaryotic cells. These proteins are intimately involved in functions such as cell and intracellular organelle movement, maintenance of cell shape and endocytosis. The cytoskeleton of most eukaryotic cells is comprised of at least three fibrous systems each with accessory proteins: the microfilaments, composed of actin; the microfibrils, composed of tubulin; and the intermediate filaments composed of vimentin. Monoclonal antibodies have been used to study the components of the cytoskeleton and their intracellular location, changes in the expression of these elements during differentiation, and the function of the cytoskeleton in living cells by microinjection of the antibodies.

Research Area

REFERENCE
REVIEW
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