ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. Optimal dilutions/concentrations should be determined by the end user.
N-terminal structural domain of b-tubulin of wide variety of species. TU-06 reacts with all charge variants of tubulin.
Phosphate buffered saline pH 7.4 containing 15mM sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Beta-subunits of tubulin (purified) (Pig).
Purified by precipitation methods
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
P07437, OK/SWcl.56, M40, MGC16435, TUBB5, 191130, TUBB, beta Tubulin, OK/SW-cl.56, MGC117247, TUBB1, 203068, OK/SW cl.56
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.
GTX27792 WB Image
Western Blotting analysis (reducing conditions) of HPB-ALL human peripheral blood leukemia cell line.
Lane 1: negative control.
Lane 2,3,4,5,6: immunostaining with anti-beta-tubulin (TU-06 (GTX27792); dilution 0,5 μg/ml, 1 μg/ml, 2 μg/ml, 4 μg/ml, 5 μg/ml)
GTX27792 ICC/IF Image
Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-beta-tubulin (TU-06) (GTX27792) (detection by Goat anti-mouse IgM Cy®5). Nucleus is stained with DAPI (blue).