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beta Tubulin antibody [TU-06]

Anti-beta Tubulin antibody [TU-06] used in Western Blot (WB). GTX27792
Anti-beta Tubulin antibody [TU-06] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27792

Cat No. GTX27792

Host Mouse
Clonality Monoclonal
Clone Name TU-06
Isotype IgM
Application WB, ICC/IF, IHC-P, IP, ELISA
Reactivity Human, Mouse, Rat, Bovine, Hamster, Pig, Carp
Package
100 μg ($319)
APPLICATION

Application Note

ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

N-terminal structural domain of b-tubulin of wide variety of species. TU-06 reacts with all charge variants of tubulin.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

Beta-subunits of tubulin (purified) (Pig).

Purification

Purified by precipitation methods

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P07437, OK/SWcl.56, M40, MGC16435, TUBB5, 191130, TUBB, beta Tubulin, OK/SW-cl.56, MGC117247, TUBB1, 203068, OK/SW cl.56

Cellular Localization

Cytoplasmic

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Research Area

DATA IMAGES
Anti-beta Tubulin antibody [TU-06] used in Western Blot (WB). GTX27792

GTX27792 WB Image

Western Blotting analysis (reducing conditions) of HPB-ALL human peripheral blood leukemia cell line.
Lane 1: negative control.
Lane 2,3,4,5,6: immunostaining with anti-beta-tubulin (TU-06 (GTX27792); dilution 0,5 μg/ml, 1 μg/ml, 2 μg/ml, 4 μg/ml, 5 μg/ml)

Anti-beta Tubulin antibody [TU-06] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27792

GTX27792 ICC/IF Image

Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-beta-tubulin (TU-06) (GTX27792) (detection by Goat anti-mouse IgM Cy®5). Nucleus is stained with DAPI (blue).

REFERENCE
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REVIEW
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