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beta Tubulin antibody

Orthogonal
Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Anti-beta Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX101279
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279
Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

Cat. No. GTX101279

Reference 36
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish, Hamster
Package
100 μl ($399),
25 μl ($169)
See all beta Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Positive Control

NIH-3T3 , 293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952434

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101279

GTX101279 ICC/IF Image

beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin stained by beta Tubulin antibody (GTX101279) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX101279

GTX101279 IHC-Wm Image

beta Tubulin antibody detects beta Tubulin protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
beta Tubulin antibody (GTX101279) dilution: 1:100.
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
beta Tubulin stained by beta Tubulin antibody (GTX101279) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
beta Tubulin stained by beta Tubulin antibody (GTX101279) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
beta Tubulin antibody (GTX101279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spinal cord by immunohistochemical analysis.
Sample: Paraffin-embedded rat spinal cord.
beta Tubulin antibody (GTX101279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

GTX101279 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta Tubulin antibody (GTX101279) at 1:300 dilution.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
beta Tubulin antibody (GTX101279) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101279

GTX101279 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using beta Tubulin(GTX101279) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101279 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

beta Tubulin antibody detects beta Tubulin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2018. PMID: 29950685

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

The data was published in the journal Cell Biosci in 2014. PMID: 25126410

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

The data was published in the journal Cancer Metab in 2014. PMID: 25225614

REFERENCE

Application Reference

M Yoshimoto et al. Exp Cell Res 2024; 437 (2) : 114018 Alterations of UHRF family Expression and was regulated by High Risk Type HPV16 in Uterine Cervical Cancer
M Viettri et al. Microbiol Spectr 2023; : e0063023 Comparative Infections of Zika, Dengue, and Yellow Fever Viruses in Human Cytotrophoblast-Derived Cells Suggest a Gating Role for the Cytotrophoblast in Zika Virus Placental Invasion
Reactivity : Mouse
Cheng H et al. Evid Based Complement Alternat Med 2023; 2023 : 8673136 Study on Neuroprotective Mechanism of Houshiheisan in Ischemic Stroke Based on Transcriptomics and Experimental Verification.
Application : WB
Reactivity : Rat
Jiang B et al. Mol Med Rep 2023; 27 (2) Combination of chloroquine diphosphate and salidroside induces human liver cell apoptosis via regulation of mitochondrial dysfunction and autophagy.
Venuti A et al. J Virol 2022; 96 (14) : e0206121 Lyon IARC Polyomavirus Displays Transforming Activities in Primary Human Cells.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

beta Tubulin antibody Cat. No. GTX101279

Rating
( Average 4 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4 based on 2 users reviews)
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Date : Anonymous submitted on 04-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 20μg
Blocking : 5% skim milk in TBS, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Date : Anonymous submitted on 04-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Amount used : 20μg
Blocking : 5% skim milk in TBS, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Submit a Review
Application Reference
M Yoshimoto et al. Exp Cell Res 2024; 437 (2):114018 Alterations of UHRF family Expression and was regulated by High Risk Type HPV16 in Uterine Cervical Cancer
M Viettri et al. Microbiol Spectr 2023;:e0063023 Comparative Infections of Zika, Dengue, and Yellow Fever Viruses in Human Cytotrophoblast-Derived Cells Suggest a Gating Role for the Cytotrophoblast in Zika Virus Placental Invasion
Reactivity : Mouse
Cheng H et al. Evid Based Complement Alternat Med 2023; 2023:8673136 Study on Neuroprotective Mechanism of Houshiheisan in Ischemic Stroke Based on Transcriptomics and Experimental Verification.
Application : WB
Reactivity : Rat
Jiang B et al. Mol Med Rep 2023; 27 (2) Combination of chloroquine diphosphate and salidroside induces human liver cell apoptosis via regulation of mitochondrial dysfunction and autophagy.
Peng KL et al. Proc Natl Acad Sci U S A 2022; 119 (37):e2203738119 Miat and interacting protein Metadherin maintain a stem-like niche to promote medulloblastoma tumorigenesis and treatment resistance.
Chen GT et al. Mol Cancer Res 2022; 20 (3):468-484 Disruption of β-Catenin-Dependent Wnt Signaling in Colon Cancer Cells Remodels the Microenvironment to Promote Tumor Invasion.
Application : WB
Reactivity : Human
Venuti A et al. J Virol 2022; 96 (14):e0206121 Lyon IARC Polyomavirus Displays Transforming Activities in Primary Human Cells.
Application : WB
Reactivity : Human
Wen-Jui Lee et al. Molecules 2021; 26 (21) Type-3 Hyaluronan Synthase Attenuates Tumor Cells Invasion in Human Mammary Parenchymal Tissues.
Application : ICC/IF
Reactivity : Human
Huang SS et al. Front Oncol 2021; 11:638311 A Novel Invadopodia-Specific Marker for Invasive and Pro-Metastatic Cancer Stem Cells.
Application : WB
Reactivity : Human
Kim S et al. Cell Death Differ 2021; PARsylated transcription factor EB (TFEB) regulates the expression of a subset of Wnt target genes by forming a complex with β-catenin-TCF/LEF1.
Application : WB
Reactivity : Human
Eller C et al. Nat Commun 2020; 11 (1):2707 A genome-wide gain-of-function screen identifies CDKN2C as a HBV host factor.
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
Application : WB
Reactivity : Human
George T. Chen et al. bioRxiv 2019; (Epub) Disrupting ?-catenin dependent Wnt signaling activates an invasive gene program predictive of colon cancer progression.
Application : WB
Reactivity : Human
Ishida Y et al. J Interferon Cytokine Res 2019; 39 (3):133-146 Hepatic IFN-Induced Protein with Tetratricopeptide Repeats Regulation of HCV Infection.
Application : WB
Reactivity : Human
Pai VC et al. Oncogene 2018; (Epub) ASPM promotes prostate cancer stemness and progression by augmenting Wnt-Dvl-3-β-catenin signaling.
Application : WB
Reactivity : Human
Wakae K et al. Sci Rep 2018; 8 (1):9745 Keratinocyte differentiation induces APOBEC3A, 3B, and mitochondrial DNA hypermutation.
Application : WB
Reactivity : Human
Lin M et al. Phytother Res 2018; (Epub) Quercetin improves postpartum hypogalactia in milk-deficient mice via stimulating prolactin production in pituitary gland.
Application : WB
Reactivity : Mouse
Yoshimoto M et al. Oncology 2018; (Epub) Abnormal Expression of PICT-1 and Its Codon 389 Polymorphism Is a Risk Factor for Human Endometrial Cancer.
Application : WB
Reactivity : Human
Wang F et al. Mol Cancer Ther 2018; 17 (4):763-775 SKLB-23bb, A HDAC6-Selective Inhibitor, Exhibits Superior and Broad-Spectrum Antitumor Activity via Additionally Targeting Microtubules.
Application : WB
Reactivity : Human
Yu-Lee LY et al. Cancer Res 2018; (Epub) Osteoblast-secreted factors mediate dormancy of metastatic prostate cancer in the bone via activation of the TGFβRIII-p38MAPK-pS249/T252RB pathway.
Application : WB
Reactivity : Human
Her LS et al. Theranostics 2017; 7 (9):2452-2462 miR-196a Enhances Neuronal Morphology through Suppressing RANBP10 to Provide Neuroprotection in Huntington's Disease.
Application : ICC/IF
Reactivity : Mouse
Cheng JC et al. Sci Rep 2017; 7 (1):4448 Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes.
Application : WB
Reactivity : Rat
Chiang WC et al. Proc Natl Acad Sci U S A 2017; 114 (2):400-405 Achromatopsia mutations target sequential steps of ATF6 activation.
Application : WB
Reactivity : Human
Yoshimoto M et al. Mol Carcinog 2016; (Epub) The Protein Interacting with Carboxyl Terminus-1 codon 389 polymorphism impairs protein interacting with carboxyl terminus-1 function and is a risk factor for uterine cervical cancer.
Albert JS et al. Orphanet J Rare Dis 2015; 10:27 Impaired osteoblast and osteoclast function characterize the osteoporosis of Snyder - Robinson syndrome.
Application : WB
Reactivity : Human
Hsiao WY et al. PLoS One 2015; 10 (12):e0145880 Dual-Specificity Phosphatase 4 Regulates STAT5 Protein Stability and Helper T Cell Polarization.
Application : WB
Reactivity : Human
Kohl S et al. Nat Genet 2015; 47 (7):757-65 Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia.
Lin YJ et al. Biomedicine (Taipei) 2015; 5 (2):10 P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions.
Application : WB
Reactivity : Hamster
Murakami S et al. Neurochem Res 2015; 40 (6):1165-78 Long-Term Systemic Exposure to Rotenone Induces Central and Peripheral Pathology of Parkinson's Disease in Mice.
Application : IHC-P
Reactivity : Mouse
Lee YZ et al. Oncotarget 2014; 5 (15):6087-101 Discovery of selective inhibitors of Glutaminase-2, which inhibit mTORC1, activate autophagy and inhibit proliferation in cancer cells.
Application : WB
Reactivity : Human
Roy D et al. Cancer Metab 2014; 2:13 Loss of HSulf-1 promotes altered lipid metabolism in ovarian cancer.
Application : WB
Reactivity : Human
Lin YJ et al. Cell Biosci 2014; 4:40 Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture.
Law CH et al. Toxicology 2013; 303:54-71 Hyaluronic acid-dependent protection in H9C2 cardiomyocytes: a cell model of heart ischemia-reperfusion injury and treatment.
Application : WB
Reactivity : Rat
Fan CY et al. Electrophoresis 2013; 34 (24):3305-14 Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Application : WB
Reactivity : Human
Lin ST et al. J Proteomics 2012; 75 (18):5822-47 proteomic analysis of proteins responsible for the development of * resistance in human uterine cancer cells.
Application : WB
Reactivity : Human
Cho CK et al. J Proteome Res 2010; 9 (7):3574-82 Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169