APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , 293T
Predict Reactivity
Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11163198
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
M40 antibody , MGC117247 antibody , MGC16435 antibody , OK/SW-cl.56 antibody , TUBB1 antibody , TUBB5 antibody , TUBB antibody , beta1-tubulin antibody , beta-4 tubulin antibody , tubulin beta-1 chain antibody , tubulin beta chain antibody , tubulin beta polypeptide antibody , "tubulin , beta polypeptide antibody" , class I beta-tubulin antibody , beta Ib tubulin antibody , beta 5-tubulin antibody , tubulin beta-5 chain antibody , "tubulin , beta antibody"
Research Area
DATA IMAGES
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GTX112659 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using beta Tubulin(GTX112659) antibody at 1:500 dilution.
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GTX112659 WB Image
Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX112659 diluted at 1:1000
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GTX112659 IHC-P Image
Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using beta Tubulin(GTX112659) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112659 WB Image
Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
10% SDS PAGE
GTX112659 diluted at 1:10000
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GTX112659 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
D: C2C12
E: Raw264.7
10% SDS PAGE
GTX112659 diluted at 1:1000
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REFERENCE
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REVIEW
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