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beta Tubulin antibody

Anti-beta Tubulin antibody used in Western Blot (WB). GTX112659
Anti-beta Tubulin antibody used in Western Blot (WB). GTX112659
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112659
Anti-beta Tubulin antibody used in Western Blot (WB). GTX112659
Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112659

Cat No. GTX112659

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A549 , NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

M40 antibody, MGC117247 antibody, MGC16435 antibody, OK/SW-cl.56 antibody, TUBB1 antibody, TUBB5 antibody, TUBB antibody, beta1-tubulin antibody, beta-4 tubulin antibody, tubulin beta-1 chain antibody, tubulin beta chain antibody, tubulin beta polypeptide antibody, "tubulin, beta polypeptide antibody", class I beta-tubulin antibody, beta Ib tubulin antibody, beta 5-tubulin antibody, tubulin beta-5 chain antibody, "tubulin, beta antibody"

Research Area

DATA IMAGES
Anti-beta Tubulin antibody used in Western Blot (WB). GTX112659

GTX112659 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX112659 diluted at 1:1000

Anti-beta Tubulin antibody used in Western Blot (WB). GTX112659

GTX112659 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
10% SDS PAGE
GTX112659 diluted at 1:10000

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112659

GTX112659 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using beta Tubulin(GTX112659) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Tubulin antibody used in Western Blot (WB). GTX112659

GTX112659 WB Image

Sample (30 ug of whole cell lysate) 
      A: NIH-3T3
      B: JC
C: BCL-1
      D: C2C12
      E: Raw264.7
      10% SDS PAGE 
      GTX112659 diluted at 1:1000

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112659

GTX112659 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using beta Tubulin(GTX112659) antibody at 1:500 dilution.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf