ICC/IF analysis of HeLa cells using GTX25794 c-Fos antibody.
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 1:500 for at least 1 hour at room temperature

WB analysis of whole cell extracts (30 μg lysate) of HEL 92.1.7 (Lane 1), Jurkat (Lane 2), HeLa (Lane 3), HeLa treated for 20 minutes with 200 mM of TPA (Lane 4), K562 (Lane 5), KARPAS-299 (Lane 6) and HT-29 (Lane 7) using GTX25794 c-Fos antibody.
Dilution : 1-3 μg/ml

WB analysis of 50 ug of serum (20% Serum, 2 hours following 0.25% serum starvation, 36 hours) treated HeLa and 293T whole cell lysate using GTX25794 c-Fos antibody.
Dilution : 1:500