IHC-P analysis of human breast carcinoma tissue using GTX17933 c-Fos (phospho Thr232) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

ICC/IF analysis of HeLa cells treated with 20 ng of EGF for 10 minutes using GTX17933 c-Fos (phospho Thr232) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

ChIP analysis of 2 million A431 cells treated with EGF (200ng/ml) for 30 minutes using GTX17933 c-Fos (phospho Thr232) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of IL-6, MMP9 gene, used as positive control target, and the SAT2 used as negative control target.

WB (peptide competition) analysis of SKBR3 cells treated with 200ng/mL EGF for 10 minutes using GTX17933 c-Fos (phospho Thr232) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.