WB (peptide competition) analysis of M07e cells unstimulated (Lane 1) or stimulated with 250ng/mL SCF (Lane 2-5) using GTX25634 c-Kit (phospho Tyr823) antibody with non-phosphorylated peptide (Lane 3), generic phosphotyrosine-containing peptide (Lane 4), phosphopeptide immunogen (Lane 5) . The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

ICC/IF analysis of Jurkat cells treated with 250 ng/ml SCF for 5 minutes using GTX25634 c-Kit (phospho Tyr823) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes