GeneTex
  • Country / Location Selection

United States (US)

c-Met antibody [3i20(25H2)]

Anti-c-Met antibody [3i20(25H2)] used in Western Blot (WB). GTX14700

Cat No. GTX14700

Host

Mouse

Clonality

Monoclonal

Clone Name

3i20(25H2)

Isotype

IgG1

Application

WB, IP

Reactivity

Human, Mouse, Rat, Monkey
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
IP 1:50
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Product Note

Recognizes endogenous levels of human Met at ~145kD and at 170kD for pro-Met.

PROPERTIES

Form

Liquid

Buffer

10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues surrounding Tyr1234 of human Met.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AUTS9 , DFNB97 , HGFR , MET , MET protooncogene, receptor tyrosine kinase , RCCP2 , cMet , hepatocyte growth factor receptor , C-Met , MET proto-oncogene, receptor tyrosine kinase

Cellular Localization

Membrane,Secreted

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]

Database

Research Area

DATA IMAGES

Anti-c-Met antibody [3i20(25H2)] used in Western Blot (WB). GTX14700

GTX14700 WB Image

WB analysis of various samples using GTX14700 c-Met antibody [3i20(25H2)].
Lane 1 : mIMCD3
Lane 2 : Vero
Lane 3 : 293 (HGF stimulated)
Lane 4 : 293 (starved)
Lane 5 : C6

REFERENCE

There are currently no references for c-Met antibody [3i20(25H2)] (GTX14700). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for c-Met antibody [3i20(25H2)] (GTX14700). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf