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c-Met antibody [3i20(25H2)]

Orthogonal
Anti-c-Met antibody [3i20(25H2)] used in Western Blot (WB). GTX14700

Cat. No. GTX14700

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3i20(25H2)

Isotype

 IgG1

Application

 WB, IP, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat, Monkey, Macaque
Package
50 μl ($399)
See all c-Met products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IP 1:50
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Product Note

Recognizes endogenous levels of human Met at ~145kD and at 170kD for pro-Met.

PROPERTIES

Form

Liquid

Buffer

10mM Sodium HEPES pH7.5, 150mM Sodium Chloride, 0.1mg/ml BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues surrounding Tyr1234 of human Met.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AUTS9 , DFNB97 , HGFR , MET , MET protooncogene, receptor tyrosine kinase , RCCP2 , cMet , hepatocyte growth factor receptor , C-Met , MET proto-oncogene, receptor tyrosine kinase

Cellular Localization

Membrane,Secreted

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]

Database

Research Area

DATA IMAGES

Anti-c-Met antibody [3i20(25H2)] used in Western Blot (WB). GTX14700

GTX14700 WB Image

Orthogonal

WB analysis of various samples using GTX14700 c-Met antibody [3i20(25H2)].
Lane 1 : mIMCD3
Lane 2 : Vero
Lane 3 : 293 (HGF stimulated)
Lane 4 : 293 (starved)
Lane 5 : C6

REFERENCE

Clone Reference

Matthew C. Judson et al. Cereb Cortex 2011; 21 (7) Conserved subcortical and divergent cortical expression of proteins encoded by orthologs of the autism risk gene MET
Application : IHC (Free Floating)
Reactivity : Mouse, Macaque
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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399