GeneTex
United States (US)

c-Met antibody

Cat No. GTX10681

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA, IHC, Neut
Reactivity Mouse
APPLICATION

Application Note

ELISA: Use at a concentration of 0.5 - 1 μg/ml. The detection limit for recombinant mouse HGF R is approximately 0.3 ng/well. Neut: Use at a concentration of 0.3 - 1 μg/ml. WB: Use at a concentration of 0.1 - 0.2 μg/ml. The detection limit for recombinant mouse HGF R is approximately 25 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 87 kDa. Anti-Mouse Hepatocyte Growth Factor Receptor has the ability to neutralize receptor-ligand interaction. Approximately 0.3-1.0 μg/ml of the antibody will block 50% of the binding of recombinant human HGF (5 ng/ml) to immobilized recombinant mouse HGF R/Fc chimera (100 μl of a 1 μg/ml solution coated in each well) in an ELISA. Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

Anti-Mouse Hepatocyte Growth Factor Receptor will neutralize receptor-ligand interaction. By ELISA, the antibody shows approximately 15% cross-reactivity with recombinant human HGF R and no cross-reactivity with recombinant human macrophage stimulating protein receptor (MSP R).
PROPERTIES

Form

Liquid

Buffer

Preservative: None Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant mouse hepatocyte growth factor receptor (HGF R) extracellulardomain expressed in Sf 21 cells.

Purification

Immunogen affinity purified
The antibody is purified using mouse HGF R affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Cell Membrane

Background

Hepatocyte growth factor receptor (HGF R), a product of the proto-oncogene c-Met, is a heterodimeric transmembrane glycoprotein that is a receptor-type tyrosine kinase. The c-Met heterodimer is composed of an alpha chain that is disulfide-linked to a beta chain. Each alpha and beta subunit heterodimer contain 1152 amino acid residues with a calculated molecular mass of approximately 129 kDa. The alpha chain is exposed to the cell surface and the beta chain spans the plasma membrane. c-Met is synthesized as a single-chain precursor which undergoes cotranslational glycosylation and proteolytic cleavage producing the heterodimeric mature form. Human and mouse HGF receptors share 89% amino acid identity. HGF is the ligand for the HGF receptor. Human HGF can bind to the mouse HGF receptor.Hepatocyte growth factor (HGF), also known as scatter factor (SF), is a multifunctional cytokine that promotes mitogenesis, migration, invasion, and morphogenesis. HGF stimulates hepatocytes and other epithelial and endothelial cells by various biological actions. HGF binding involves the beta chain of the HGF receptor, but alpha chain participation cannot be ruled out. HGF binding to c-Met triggers dimerization and subsequent tyrosine autophosphorylation of the receptor beta chain. Autophophorylation at two tyrosines upregulates kinase activity while phosphorylation at two other tyrosines generates SH2 docking sites for adapter proteins such as Shc, Grb2, CrK/CRKL, and Gab1. Receptor activation has been correlated to the activation of the Ras pathway, which culminates in the activation and consequent nuclear translocation of MAP kinase. c-Met can also be negatively modulated by phosphorylation of Ser 985 by protein kinase C. Other ligand-receptor activities involve binding that leads to enhanced integrin-mediated B cell and lymphoma cell adhesion. Normal HGF-Met signaling is needed for embryonic development and abnormal signaling and has been implicated in tumorigenesis.

Research Area

Package List Price ($)
$ 319