APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| Assay dependent |
| 1:20-1:200 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 1mg/ml BSA, 50% Glycerol
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human c-Met that contains tyrosine 1003. The sequence is conserved in mouse and rat.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
MET proto-oncogene, receptor tyrosine kinase , AUTS9 , DFNB97 , HGFR , RCCP2 , c-Met
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]
Database
Research Area
DATA IMAGES
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GTX25656 WB Image
WB analysis of H69 cells unstimulated (Lane 1) or treated with 40 ng/mL HGF for 7.5 minutes (Lanes 2-5) using GTX25656 c-Met (phospho Tyr1003) antibody.
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GTX25656 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX25656 c-Met (phospho Tyr1003) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100
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REFERENCE
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REVIEW
There are currently no reviews for c-Met (phospho Tyr1003) antibody (GTX25656). Be the first to share your experience with this product.
