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c-Met (phospho Tyr1003) antibody

Anti-c-Met (phospho Tyr1003) antibody used in IHC (Paraffin sections) (IHC-P). GTX25656
Anti-c-Met (phospho Tyr1003) antibody used in Western Blot (WB). GTX25656

Cat No. GTX25656

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC

Reactivity

Human, Mouse, Dog, Hamster
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1:20-1:200
IHC Assay dependent
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1mg/ml BSA, 50% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human c-Met that contains tyrosine 1003. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MET proto-oncogene, receptor tyrosine kinase , AUTS9 , DFNB97 , HGFR , RCCP2 , c-Met

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]

Database

Research Area

DATA IMAGES

Anti-c-Met (phospho Tyr1003) antibody used in IHC (Paraffin sections) (IHC-P). GTX25656

GTX25656 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX25656 c-Met (phospho Tyr1003) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-c-Met (phospho Tyr1003) antibody used in Western Blot (WB). GTX25656

GTX25656 WB Image

WB analysis of H69 cells unstimulated (Lane 1) or treated with 40 ng/mL HGF for 7.5 minutes (Lanes 2-5) using GTX25656 c-Met (phospho Tyr1003) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf