APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1-2ug/ml |
| 1-2ug/ml for 30 minutes at RT |
|
Note :
IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
|
Not tested in other applications.
Calculated MW
Specificity/Sensitivity
It recognizes a transcription factor of 64-67kDa, identified as c-myc. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or over-expressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease. Rearrangement of the MYC gene is found in 3% to 16% of diffuse large B-cell lymphoma (DLBCLā€™s) and in nearly 100% of Burkitt lymphomas (BL). Identifying MYC status is important in establishing final diagnosis of DLBCL, BL, or B-cell lymphoma, with features intermediate between DLBCL and BL as well as in differential diagnoses of the lymphomas.
PROPERTIES
Form
Liquid
Buffer
10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml
Antigen Species
Human
Immunogen
Recombinant human c-myc protein
Purification
Ab purified from Bioreactor Concentrate by Protein A/G
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Myc Proto-Oncogene, Bhlh Transcription Factor , Mrtl , Mycc , Bhlhe39 , C-Myc , Myc
Background
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-c-Myc antibody [MYC909] used in IHC (Paraffin sections) (IHC-P). GTX34612](/upload/website/prouct_img/middle/GTX34612/GTX34612_20170712_IHC-P_18121410_777.jpg "Anti-c-Myc antibody [MYC909] used in IHC (Paraffin sections) (IHC-P). GTX34612")
|
GTX34612 IHC-P Image
Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with c-myc Monoclonal Antibody (MYC909).
|
REFERENCE
There are currently no references for c-Myc antibody [MYC909] (GTX34612). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for c-Myc antibody [MYC909] (GTX34612). Be the first to share your experience with this product.
![Anti-c-Myc antibody [MYC909] used in IHC (Paraffin sections) (IHC-P). GTX34612](/upload/website/prouct_img/small/GTX34612/GTX34612_20170712_IHC-P_18121410_777.jpg "Anti-c-Myc antibody [MYC909] used in IHC (Paraffin sections) (IHC-P). GTX34612")
![Anti-c-Myc antibody [MYC909] used in IHC (Paraffin sections) (IHC-P). GTX34612](/upload/website/prouct_img/normal/GTX34612/GTX34612_20170712_IHC-P_18121410_777.jpg "Anti-c-Myc antibody [MYC909] used in IHC (Paraffin sections) (IHC-P). GTX34612")
