GeneTex

  • Country / Location Selection

United States (US)

c-Myc antibody [MYC909]

Anti-c-Myc antibody [MYC909] used in IHC (Paraffin sections) (IHC-P). GTX34612

Cat No. GTX34612

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MYC909

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($339)
See all c-Myc products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes a transcription factor of 64-67kDa, identified as c-myc. This MAb shows no cross-reaction with v-myc.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human c-myc protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MRTL , MYC , MYCC , bHLHe39 , cMyc , vmyc avian myelocytomatosis viral oncogene homolog , cmyc , MYC proto-oncogene, bHLH transcription factor

Cellular Localization

Nucleus,Nucleoplasm,Nucleolus

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-c-Myc antibody [MYC909] used in IHC (Paraffin sections) (IHC-P). GTX34612

GTX34612 IHC-P Image

IHC-P analysis of human cervical carcinoma tissue using GTX34612 c-Myc antibody [MYC909].

REFERENCE

There are currently no references for c-Myc antibody [MYC909] (GTX34612). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for c-Myc antibody [MYC909] (GTX34612). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339