WB analysis of whole cell extracts (30 μg lysate) of A549 (Lane 1), Hep G2 (lane 2) and PANC-1 (Lane 3) using GTX54748 eIF1 antibody [2B9].
Dilution : 1:500-1:2000

ICC/IF analysis of MCF-7 cells using GTX54748 eIF1 antibody [2B9]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton„ X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

ICC/IF analysis of HeLa cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

ICC/IF analysis of NIH-3T3 cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

WB analysis of 80μg whole cell lysate using GTX54748 eIF1 antibody [2B9].
Dilution : 1:1000

ICC/IF analysis of MCF-7 cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

ICC/IF analysis of HeLa cells and NIH3T3 cells using GTX54748 eIF1 antibody [2B9].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature