GeneTex
United States (US)

eNOS (phospho Ser1177) antibody

Orthogonal
GTX129058_41423_20180105_WB_18121410_371.jpg
GTX129058_43019_20171121_IHC-P_R_18121410_544.jpg
GTX129058_41423_20180105_WB_treatment_PMA_18121410_270.jpg

Cat No. GTX129058

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Positive Control

Raji , NCI-H929 , THP-1 , THP-1 (100 nM PMA treatment for 6, 24, 48 hr)

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human eNOS (phospho Ser1177). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NOS3 Antibody, nitric oxide synthase 3 (endothelial cell) Antibody, eNOS Antibody, ECNOS Antibody

Cellular Localization

Cell membrane , Membrane , caveola , Cytoplasm , cytoskeleton , Golgi apparatus

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX129058_41423_20180105_WB_18121410_371.jpg

GTX129058 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129058_43019_20171121_IHC-P_R_18121410_544.jpg

GTX129058 IHC-P Image

eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX129058_41423_20180105_WB_treatment_PMA_18121410_270.jpg

GTX129058 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf