Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Raji , NCI-H929 , THP-1 , THP-1 (100 nM PMA treatment for 6, 24, 48 hr)
Predict Reactivity
Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig(>80% identity)
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide corresponding to residues around human eNOS (phospho Ser1177). The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
NOS3 Antibody, nitric oxide synthase 3 (endothelial cell) Antibody, eNOS Antibody, ECNOS Antibody
Cellular Localization
Cell membrane , Membrane , caveola , Cytoplasm , cytoskeleton , Golgi apparatus
Background
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
