eNOS (phospho Ser1177) antibody

Name: eNOS (phospho Ser1177) antibody
Brand: GeneTex
SKU: GTX129058
Price: 379.00 USD
Rating: 5 (1 reviews)

Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX129058

Anti-eNOS (phospho Ser1177) antibody used in IHC (Paraffin sections) (IHC-P). GTX129058

Anti-eNOS (phospho Ser1177) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129058

Cat No. GTX129058

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($379), 25 μl ($169)

See all eNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Positive Control

Raji , NCI-H929 , THP-1 , THP-1 (100 nM PMA treatment for 6, 24, 48 hr)

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser1177 of human eNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885882

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nitric oxide synthase 3 , ECNOS , eNOS

Cellular Localization

Cell membrane , Membrane , caveola , Cytoplasm , cytoskeleton , Golgi apparatus

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 4846 NOS3
UniProt: P29474 NOS3

Research Area

DATA IMAGES

GTX129058 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129058 IHC-P Image

eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX129058 WB Image

Whole cell extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129058 IHC-P Image

eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX129058 ICC/IF Image

eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX129058 WB Image

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129058 IHC-P Image

eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Garc?a-Arroyo FE et al. Biomolecules 2019; 9 (10) Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats.

Fan Y et al. BMC Complement Altern Med 2019; 19 (1) : 355 Astragaloside IV protects against diabetic nephropathy via activating eNOS in streptozotocin diabetes-induced rats.

Yamada T et al. Circ J 2018; 83 (1) : 232-238 Preserved Vasoconstriction and Relaxation of Saphenous Vein Grafts Obtained by a No-Touch Technique for Coronary Artery Bypass Grafting.

Application : IHC-P

Reactivity : Human

Papinska AM et al. Oxid Med Cell Longev 2018; 2018 : 1841046 Long-term administration of angiotensin (1-7) to db/db mice reduces oxidative stress damage in the kidneys and prevents renal dysfunction.

Application : IHC-P

Reactivity : Mouse

Ann P Lawson et al. Oncotarget 2017; 8 (31) : 51296-51316 Identification of deubiquitinase targets of isothiocyanates using SILAC-assisted quantitative mass spectrometry.

Application : WB

Reactivity : Rat

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