APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Raji , NCI-H929 , THP-1 , THP-1 (100 nM PMA treatment for 6, 24, 48 hr)
Predict Reactivity
Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.74 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide corresponding to residues around human eNOS (phospho Ser1177). The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
nitric oxide synthase 3 , ECNOS , eNOS
Cellular Localization
Cell membrane , Membrane , caveola , Cytoplasm , cytoskeleton , Golgi apparatus
Background
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX129058 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX129058 IHC-P Image
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX129058 IHC-P Image
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX129058 WB Image
Whole cell extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX129058 WB Image
Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX129058 IHC-P Image
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
REFERENCE
REVIEW
There are currently no reviews for eNOS (phospho Ser1177) antibody (GTX129058). Be the first to share your experience with this product.
![GeneTex Launches Novel Recombinant RAS (G12D Mutant) Antibody [HL10]](/upload/website/marketing_material/list_image/genetex_20200212_KRAS_slide_255x170_20021120_758.jpg "GeneTex Launches Novel Recombinant RAS (G12D Mutant) Antibody [HL10]")
