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gamma Tubulin antibody

Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11321
Anti-gamma Tubulin antibody used in Western Blot (WB). GTX11321
Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11321

Cat No. GTX11321

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat, Dog, Frog
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5,000
ICC/IF 1:1,000
Not tested in other applications.

Positive Control

Xenopus eggs, Rat2

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Xenopus laevis

Immunogen

synthetic peptide corresponding to the C-terminus of Xenopus γ-tubulin (amino acids 437-451 with N-terminal added cys-gly), conjugated to KLH.

Purification

IgG fraction

Conjugation

Unconjugated

RRID

AB_384220

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin gamma 1 L homeolog , Tubg2 , tubg1 , xgam

Background

The protein that binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome is called gamma tubulin. Gamma Tubulin functions as the microtubule nucleator at the microtubule organizing centre (MTOC). By binding to the beta tubulin half of the tubulin molecule, it establishes the polarity of a microtubule, leaving the alpha tubulin half exposed at the positive end. Gamma Tubulin (approx. 48 kD) is a ubiquitous and highly conserved protein within the MTOCs in eukaryotic kingdom. It is related to alpha and beta tubulin and is, thus, a member of the tubulin superfamily of proteins. However, its abundance is less than 1% of the level of either alpha or beta tubulin. Moreover, unlike alpha and beta tubulin, it is not a component of microtubules. Rather, it is located at the MTOC. gamma Tubulin shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulin serve as an essential tool in the detection of the presence and role of this molecule in various cellular settings.

Database

Research Area

DATA IMAGES

Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11321

GTX11321 ICC/IF Image

ICC/IF analysis of Rat1 cells using GTX11321 gamma Tubulin antibody at 1:1,000. Cells were fixed and permeabilized with methanol followed by acetone.

Anti-gamma Tubulin antibody used in Western Blot (WB). GTX11321

GTX11321 WB Image

WB analysis of (1) HeLa (2) NIH-3T3 (3) MDCK (4) CHO (5) rat2 (6) A431 (7) JURKAT (8) U87 (9) C2C12 cells using GTX11321 gamma Tubulin antibody at 1:1,000.

Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11321

GTX11321 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX11321 gamma Tubulin antibody at 1:1,000. Cells were fixed and permeabilized with methanol followed by acetone.

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379