0.01M PBS pH7.4
15mM Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
synthetic peptide corresponding to the C-terminus of Xenopus γ-tubulin (amino acids 437-451 with N-terminal added cys-gly), conjugated to KLH.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
tubulin gamma 1 L homeolog , Tubg2 , tubg1 , xgam
The protein that binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome is called gamma tubulin. Gamma Tubulin functions as the microtubule nucleator at the microtubule organizing centre (MTOC). By binding to the beta tubulin half of the tubulin molecule, it establishes the polarity of a microtubule, leaving the alpha tubulin half exposed at the positive end. Gamma Tubulin (approx. 48 kD) is a ubiquitous and highly conserved protein within the MTOCs in eukaryotic kingdom. It is related to alpha and beta tubulin and is, thus, a member of the tubulin superfamily of proteins. However, its abundance is less than 1% of the level of either alpha or beta tubulin. Moreover, unlike alpha and beta tubulin, it is not a component of microtubules. Rather, it is located at the MTOC. gamma Tubulin shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulin serve as an essential tool in the detection of the presence and role of this molecule in various cellular settings.