APPLICATION
Application Note
IHC: This antibody may be diluted to a titer of 1:100-1:500 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Although not required, proteolytic treatment may enhance the immunostaining in overfixed tissues.
Specificity/Sensitivity
This antibody reacts with hCG. It stains strongly with the human placenta and placental trophoblasts. No non-specific stainingwith any other tissue has been observed.
PROPERTIES
Form
Liquid
Buffer
Phosphate-buffered saline, pH 7.2, containing 0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Antigen Species
Human
Immunogen
Beta chain of human chorionic gonadotropin.
Purification
Affinity purified with antigen
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Q8WXL1, 1082, CG, hCGB, CGB, 118860, CGB3
Cellular Localization
Secreted
Background
Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium.
