GeneTex
United States (US)

hnRNP A1 antibody [4B10]

Cat No. GTX10685

Host Mouse
Clonality Monoclonal
Clone Name 4B10
Isotype IgG2a
Application WB, ICC/IF, IP, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

39 kDa. ( Note )

Specificity/Sensitivity

Human hnRNP-A1. Detects a band of approximately 34kDa. Does cross react with mouse for IP & WB
PROPERTIES

Form

Liquid

Buffer

PBS + 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

HeLa hnRNP-A1 (partially purified)

Purification

Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Helix destabilizing protein, Heterogeneous nuclear ribonucleoprotein A1, hnRNP core protein A1, HNRNPA1, MGC102835, Heterogeneous nuclear ribonucleoprotein A1B protein, Heterogeneous nuclear ribonucleoprotein B2 protein, Heterogeneous nuclear ribonucleoprotein core protein A1, HNRPA1, Nuclear ribonucleoprotein particle A1 protein, Single strand DNA binding protein UP1, Single strand RNA binding protein, Syncrip,

Cellular Localization

Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the

Background

This protein belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre mRNAs in the nucleus and appear to influence pre mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. hnRNP A1 has two repeats of quasi RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought to have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for the hnRNP A1 gene but only two transcripts are fully described. These variant have multiple alternative transcription initiation sites and multiple polyA sites.

Database

Research Area

Package List Price ($)
$ 319