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hnRNP AB antibody

Anti-hnRNP AB antibody used in Western Blot (WB). GTX101852
Anti-hnRNP AB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101852
Anti-hnRNP AB antibody used in IHC (Paraffin sections) (IHC-P). GTX101852
Anti-hnRNP AB antibody used in Western Blot (WB). GTX101852

Cat No. GTX101852

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP-seq
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
ChIP-seq Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP AB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein A/B , ABBP1 , HNRPAB

Cellular Localization

Nucleus

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-hnRNP AB antibody used in Western Blot (WB). GTX101852

GTX101852 WB Image

Wild-type (WT) and hnRNP AB knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP AB antibody (GTX101852) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP AB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101852

GTX101852 ICC/IF Image

hnRNP A/B antibody detects hnRNP A/B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP A/B protein stained by hnRNP A/B antibody (GTX101852) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-hnRNP AB antibody used in IHC (Paraffin sections) (IHC-P). GTX101852

GTX101852 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using HNRNPAB(GTX101852) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP AB antibody used in Western Blot (WB). GTX101852

GTX101852 WB Image

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX101852 diluted at 1:1000

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169