GeneTex
United States (US)

hnRNP AB antibody

KO/KD
GTX101852_40373_20180209_WB_KO_watermark_19011506_292.jpg
GTX101852_39645_20150525_IFA_19011506_365.jpg
GTX101852_39645_IHC_19011506_899.jpg
GTX101852_40373_WB_19011506_350.jpg

Cat No. GTX101852

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP-seq
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
ChIP-seq Assay dependent

Note :

IP
target validated by Mass Spectrometry

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP AB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ABBP1 antibody, FLJ40338 antibody, HNRPAB antibody, HNRNPAB antibody, hnRNP type A/B protein antibody, "apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1-binding protein 1 antibody", ABBP-1 antibody, heterogeneous nuclear ribonucleoprotein A/B antibody, APOBEC1-binding protein 1 antibody, hnRNP A/B antibody, apobec-1 binding protein 1 antibody

Cellular Localization

Nucleus

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101852_40373_20180209_WB_KO_watermark_19011506_292.jpg

GTX101852 WB Image

KO/KD

Wild-type (WT) and hnRNP AB knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP AB antibody (GTX101852) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101852_39645_20150525_IFA_19011506_365.jpg

GTX101852 ICC/IF Image

hnRNP A/B antibody detects hnRNP A/B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP A/B protein stained by hnRNP A/B antibody (GTX101852) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101852_39645_IHC_19011506_899.jpg

GTX101852 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using HNRNPAB(GTX101852) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101852_40373_WB_19011506_350.jpg

GTX101852 WB Image

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX101852 diluted at 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169