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hnRNP K antibody

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786
Anti-hnRNP K antibody used in Western Blot (WB). GTX101786
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786
Anti-hnRNP K antibody used in Western Blot (WB). GTX101786
Anti-hnRNP K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101786

Cat No. GTX101786

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

293T , A431 , Hela , HepG2 , Mouse brain

Predict Reactivity

Rabbit, Bovine, Dog, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein K , AUKS , CSBP , HNRPK , TUNP

Cellular Localization

Cytoplasm , Nucleus , nucleoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

GTX101786 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using hnRNP K(GTX101786) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP K antibody used in Western Blot (WB). GTX101786

GTX101786 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101786 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

GTX101786 IHC-P Image

hnRNP K antibody detects hnRNP K protein at nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
hnRNP K antibody (GTX101786) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

GTX101786 IHC-P Image

hnRNP K antibody detects hnRNP K protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
hnRNP K antibody (GTX101786) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

GTX101786 IHC-P Image

hnRNP K antibody detects hnRNP K protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
hnRNP K antibody (GTX101786) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP K antibody used in Western Blot (WB). GTX101786

GTX101786 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP K antibody (GTX101786) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101786

GTX101786 ICC/IF Image

hnRNP K antibody detects hnRNP K protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP K stained by hnRNP K antibody (GTX101786) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

REFERENCE
REVIEW

hnRNP K antibody Cat No. GTX101786

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 21-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 40μg
Blocking : 5% Skim milk in TBS+tween 0.1%, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169