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hnRNP K antibody

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786
Anti-hnRNP K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101786
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786
Anti-hnRNP K antibody used in Western Blot (WB). GTX101786
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786
Anti-hnRNP K antibody used in Western Blot (WB). GTX101786
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

Cat. No. GTX101786

Reference 4
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all hnRNP K products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , Hela , HepG2

Predict Reactivity

Rabbit, Bovine, Dog, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240956

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein K , AUKS , CSBP , HNRPK , TUNP

Cellular Localization

Cytoplasm , Nucleus , nucleoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

GTX101786 IHC-P Image

hnRNP K antibody detects hnRNP K protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
hnRNP K antibody (GTX101786) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

GTX101786 IHC-P Image

hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
hnRNP K stained by hnRNP K antibody (GTX101786) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-hnRNP K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101786

GTX101786 ICC/IF Image

hnRNP K antibody detects hnRNP K protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP K stained by hnRNP K antibody (GTX101786) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

GTX101786 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using hnRNP K(GTX101786) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP K antibody used in Western Blot (WB). GTX101786

GTX101786 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP K antibody (GTX101786) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

GTX101786 IHC-P Image

hnRNP K antibody detects hnRNP K protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
hnRNP K antibody (GTX101786) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

GTX101786 IHC-P Image

hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
hnRNP K stained by hnRNP K antibody (GTX101786) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

GTX101786 IHC-P Image

hnRNP K antibody detects hnRNP K protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
hnRNP K antibody (GTX101786) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

GTX101786 IHC-P Image

hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
hnRNP K stained by hnRNP K antibody (GTX101786) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-hnRNP K antibody used in Western Blot (WB). GTX101786

GTX101786 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101786 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX101786

GTX101786 IHC-P Image

hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
hnRNP K stained by hnRNP K antibody (GTX101786) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Ke Zhang et al. Sci Adv 2021; 7 (6) Nsp1 protein of SARS-CoV-2 disrupts the mRNA export machinery to inhibit host gene expression.
Application : ICC/IF
Reactivity : Human
Matthew G Thompson et al. Elife 2020; 9 Viral-induced alternative splicing of host genes promotes influenza replication.
Application : ICC/IF
Reactivity : Human
Chou HC et al. Life Sci 2018; (Epub) Proteomic analysis of honokiol-induced cytotoxicity in thyroid cancer cells.
Application : WB
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

hnRNP K antibody Cat. No. GTX101786

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-hnRNP K antibody used in Western Blot (WB). GTX101786
Date : Anonymous submitted on 21-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 40μg
Blocking : 5% Skim milk in TBS+tween 0.1%, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169