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hnRNP K antibody

Anti-hnRNP K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32658
Anti-hnRNP K antibody used in Western Blot (WB). GTX32658
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX32658
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX32658
Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX32658

Cat. No. GTX32658

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all hnRNP K products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HNRNPK (NP_112552.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein K , AUKS , CSBP , HNRPK , TUNP

Cellular Localization

Cytoplasm,nucleoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-hnRNP K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32658

GTX32658 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX32658 hnRNP K antibody.
Blue : DAPI

Anti-hnRNP K antibody used in Western Blot (WB). GTX32658

GTX32658 WB Image

WB analysis of various sample lysates using GTX32658 hnRNP K antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX32658

GTX32658 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX32658 hnRNP K antibody.
Dilution : 1:200

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX32658

GTX32658 IHC-P Image

IHC-P analysis of mouse esophagus tissue using GTX32658 hnRNP K antibody.
Dilution : 1:200

Anti-hnRNP K antibody used in IHC (Paraffin sections) (IHC-P). GTX32658

GTX32658 IHC-P Image

IHC-P analysis of rat liver tissue using GTX32658 hnRNP K antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399