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hnRNP K (phospho Ser284) antibody

Anti-hnRNP K (phospho Ser284) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32194
Anti-hnRNP K (phospho Ser284) antibody used in Western Blot (WB). GTX32194

Cat No. GTX32194

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of hnRNP K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein K , AUKS , CSBP , HNRPK , TUNP

Cellular Localization

Cytoplasm,nucleoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-hnRNP K (phospho Ser284) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32194

GTX32194 ICC/IF Image

ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX32194 hnRNP K (phospho Ser284) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-hnRNP K (phospho Ser284) antibody used in Western Blot (WB). GTX32194

GTX32194 WB Image

WB analysis of EGF-treated MCF7 (A), EGF-treated Raw264.7 (B), EGF-treated H9C2 (C) whole cell lysates using GTX32194 hnRNP K (phospho Ser284) antibody.

REFERENCE
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Package List Price ($)
$ 319