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hnRNP L antibody, Internal

Anti-hnRNP L antibody, Internal used in Western Blot (WB). GTX81941
Anti-hnRNP L antibody, Internal used in Western Blot (WB). GTX81941
Anti-hnRNP L antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81941
Anti-hnRNP L antibody, Internal used in Flow cytometry (FACS). GTX81941

Cat No. GTX81941

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, FACS

Reactivity

Human
Package
400 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human HNRPL.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein L , HNRPL , P/OKcl.14 , hnRNP-L

Cellular Localization

nucleoplasm,Cytoplasm

Background

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-hnRNP L antibody, Internal used in Western Blot (WB). GTX81941

GTX81941 WB Image

WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the hnRNP L (Lane 2) using GTX81941 hnRNP L antibody, Internal.

Anti-hnRNP L antibody, Internal used in Western Blot (WB). GTX81941

GTX81941 WB Image

WB analysis of HepG2, Jurkat cell lysate (35ug/lane) using GTX81941 hnRNP L antibody, Internal.

Anti-hnRNP L antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81941

GTX81941 IHC-P Image

IHC-P analysis of human hepatocarcinoma using GTX81941 hnRNP L antibody, Internal.

Anti-hnRNP L antibody, Internal used in Flow cytometry (FACS). GTX81941

GTX81941 FACS Image

FACS analysis of Jurkat cells using GTX81941 hnRNP L antibody, Internal.
Green : primary antibody
Blue : negative control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369