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hnRNP L antibody

Anti-hnRNP L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132754
Anti-hnRNP L antibody used in IHC (Paraffin sections) (IHC-P). GTX132754
Anti-hnRNP L antibody used in IHC (Paraffin sections) (IHC-P). GTX132754

Cat No. GTX132754

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

duodenum

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human hnRNP L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886730

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein L , HNRPL , P/OKcl.14 , hnRNP-L

Cellular Localization

nucleoplasm,Cytoplasm

Background

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-hnRNP L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132754

GTX132754 ICC/IF Image

hnRNP L antibody detects hnRNP L protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP L stained by hnRNP L antibody (GTX132754) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.

Anti-hnRNP L antibody used in IHC (Paraffin sections) (IHC-P). GTX132754

GTX132754 IHC-P Image

hnRNP L antibody detects hnRNP protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
hnRNP stained by hnRNP L antibody (GTX132754) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-hnRNP L antibody used in IHC (Paraffin sections) (IHC-P). GTX132754

GTX132754 IHC-P Image

hnRNP L antibody detects hnRNP protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
hnRNP stained by hnRNP L antibody (GTX132754) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169