GeneTex
United States (US)

mTOR antibody [C3], C-term

KO/KD
GTX101557_41731_20160616_WB_shRNA_watermark_19011506_638.jpg
GTX101557_39721_IHC_2_19011506_447.jpg
GTX101557_42018_20150814_IHC-P_M_19011506_537.jpg
GTX101557_41731_WB_M_brain_19011506_721.jpg
GTX101557_40555_IP_19011506_433.jpg
GTX101557_41059_IFA_19011506_168.jpg
GTX101557_40555_ChIP_19011506_128.jpg
GTX101557_41059_20150206_IHC_M_2_19011506_367.jpg
GTX101557_41731_20150910_IP_19011506_580.jpg
GTX101557_41731_20190201_WB_19022001_980.jpg

Cat No. GTX101557

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

289 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , *Flag-human mTOR transfected 293T

Predict Reactivity

Zebrafish, Sheep(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mechanistic Target Of Rapamycin Kinase,Frap,Frap1,Frap2,Raft1,Rapt1,Sks,Mtor

Cellular Localization

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101557_41731_20160616_WB_shRNA_watermark_19011506_638.jpg

GTX101557 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101557_39721_IHC_2_19011506_447.jpg

GTX101557 IHC-P Image

mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
mTOR antibody (GTX101557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101557_42018_20150814_IHC-P_M_19011506_537.jpg

GTX101557 IHC-P Image

mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101557_41731_WB_M_brain_19011506_721.jpg

GTX101557 WB Image

mTOR antibody detects mTOR protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GTX101557) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101557_40555_IP_19011506_433.jpg

GTX101557 IP Image

mTOR antibody immunoprecipitates mTOR protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of mTOR by 3 μg of mTOR antibody (GTX101557) 5% SDS-PAGE The immunoprecipitated mTOR protein was detected by mTOR antibody (GTX101557) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX101557_41059_IFA_19011506_168.jpg

GTX101557 ICC/IF Image

mTOR antibody detects mTOR protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 100% MeOH for 5 min.
Green: mTOR protein stained by mTOR antibody (GTX101557) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX101557_40555_ChIP_19011506_128.jpg

GTX101557 ChIP assay Image

Cross-linked ChIP was performed with 293T chromatin extract and 5 μg of either control rabbit IgG or anti-mTOR antibody. The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter .

GTX101557_41059_20150206_IHC_M_2_19011506_367.jpg

GTX101557 IHC-P Image

mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101557_41731_20150910_IP_19011506_580.jpg

GTX101557 IP Image

Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg of mTOR antibody [C3], C-term (GTX101557).
Western blot analysis was performed using mTOR antibody [C3], C-term (GTX101557).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101557_41731_20190201_WB_19022001_980.jpg

GTX101557 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

mTOR antibody [C3], C-term Cat No. GTX101557

Rating ( Average 4 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4 based on 3 users reviews)
Date : Anonymous submitted on 08-Sep-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : skin fibroblast tissue
Amount used : 5μg
Blocking : 1% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 03-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Flag-human mTOR transfected 293T cells
Amount used : 30μg
Blocking : 3% skim milk in PBS, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: 293T cells transfected with Flag-human mTOR. Lane 2: 293T cells.
Date : Anonymous submitted on 19-Oct-2011
Score :
Application Tested : WB
Sample Species : Rat
Sample : Neuron primary culture cells
Amount used : 100μg
Blocking : 3% non-fat milk in PBST, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169