GeneTex
United States (US)

p53 (acetyl Lys379) antibody

p53-acetyl-Lys379-antibody-GTX88013-IHC-1_18122102_117.jpg
p53-acetyl-Lys379-antibody-GTX88013-ICC_IF-1_18122102_900.jpg

Cat No. GTX88013

Host Rabbit
Clonality Polyclonal
Application ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Specificity/Sensitivity

p53 (Acetyl-Lys379) Antibody detects endogenous levels of total p53 protein only when acetylated at lysine379.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human p53 around the acetylated site of Lys379.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acet

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cellular tumor antigen p53 antibody, Tumor suppressor p53 antibody, Phosphoprotein p53 antibody, TP53 antibody, p53 antibody, BCC7 antibody, LFS1 antibody, TRP53 antibody, p53 (acetyl K379) antibody, p53 (acetyl Lys379) antibody, p53 acetyl K379 antibody, p53 acetyl Lys379 antibody, Acetyl p53 (K379) antibody, Acetyl p53 (Lys379) antibody, TP53 (acetyl K379) antibody, TP53 (acetyl Lys379) antibody, TP53 acetyl K379 antibody, TP53 acetyl Lys379 antibody, Acetyl TP53 (K379) antibody, Acetyl TP53 (Lys379) antibody, BCC7 (acetyl K379) antibody, BCC7 (acetyl Lys379) antibody, BCC7 acetyl K379 antibody, BCC7 acetyl Lys379 antibody, Acetyl BCC7 (K379) antibody, Acetyl BCC7 (Lys379) antibody, LFS1 (acetyl K379) antibody, LFS1 (acetyl Lys379) antibody, LFS1 acetyl K379 antibody, LFS1 acetyl Lys379 antibody, Acetyl LFS1 (K379) antibody, Acetyl LFS1 (Lys379) antibody, TRP53 (acetyl K379) antibody, TRP53 (acetyl Lys379) antibody, TRP53 acetyl K379 antibody, TRP53 acetyl Lys379 antibody, Acetyl TRP53 (K379) antibody, Acetyl TRP53 (Lys379) antibody

Cellular Localization

Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]
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Database

Research Area

DATA IMAGES
p53-acetyl-Lys379-antibody-GTX88013-IHC-1_18122102_117.jpg

GTX88013 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p53 (Acetyl-Lys379) Antibody. The picture on the right is treated with the synthesized peptide.

p53-acetyl-Lys379-antibody-GTX88013-ICC_IF-1_18122102_900.jpg

GTX88013 ICC/IF Image

Immunofluorescence analysis of HeLa cells, using p53 (Acetyl-Lys379) Antibody. The picture on the right is treated with the synthesized peptide.

Reference