United States (US)

p53 antibody [BP53-12]

Anti-p53 antibody [BP53-12] used in IHC (Paraffin sections) (IHC-P). GTX34937
Anti-p53 antibody [BP53-12] used in Western Blot (WB). GTX34937

Cat No. GTX34937

Host Mouse
Clonality Monoclonal
Clone Name BP53-12
Isotype IgG2a
Application WB, ICC/IF, IHC-P
Reactivity Human, Dog, Hamster, Chicken, Monkey

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

44 kDa. ( Note )


This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.




Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.


Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species



Recombinant human wild-type p53 protein


Ab purified from Bioreactor Concentrate by Protein A/G




For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


Tumor Protein P53,Bcc7,Lfs1,P53,Trp53,Tp53


This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]


Research Area

Anti-p53 antibody [BP53-12] used in IHC (Paraffin sections) (IHC-P). GTX34937

GTX34937 IHC-P Image

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with p53 Monoclonal Antibody (BP53-12).

Anti-p53 antibody [BP53-12] used in Western Blot (WB). GTX34937

GTX34937 WB Image

Western Blot of p53 1) A431; 2) MCF7; 3) HEK293 Lysate probed with p53 Monoclonal Antibody (BP53-12).

Package List Price ($)
$ 329