GeneTex
United States (US)

p53 antibody [N1], N-term

Orthogonal
GTX100629_39988_IFA_2_19011506_911.jpg
GTX100629_39988_IP_19011506_440.jpg
GTX100629_39988_IHC_19011506_925.jpg
GTX100629_39988_WB_HCT116 cisplatin_19011720_974.jpg
GTX100629_39988_IFA_19011506_811.jpg

Cat No. GTX100629

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HCT116 24hr cisplatin 30uM

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human p53. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ92943 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, TP53 antibody, cellular tumor antigen p53 antibody, antigen NY-CO-13 antibody, phosphoprotein p53 antibody, p53 tumor suppressor antibody, transformation-related protein 53 antibody, tumor protein p53 antibody

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum

Background

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100629_39988_IFA_2_19011506_911.jpg

GTX100629 ICC/IF Image

p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.
Samples: HCT 116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 protein stained by p53 antibody [N1], N-term (GTX100629) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX100629_39988_IP_19011506_440.jpg

GTX100629 IP Image

Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-p53 antibody (GTX100629). (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by GTX100629 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot.

GTX100629_39988_IHC_19011506_925.jpg

GTX100629 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using p53(GTX100629) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100629_39988_WB_HCT116 cisplatin_19011720_974.jpg

GTX100629 WB Image

Orthogonal

Sample (30 ug of whole cell lysate)
A: HCT116 cells with mock treatment for 24hr
B: HCT116 cells with 30uM cisplatin treatment for 24hr
10% SDS PAGE
GTX100629 diluted at 1:1000

GTX100629_39988_IFA_19011506_811.jpg

GTX100629 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using p53(GTX100629) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf