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p53 antibody [TRP/816]

Anti-p53 antibody [TRP/816] used in Western Blot (WB). GTX34934
Anti-p53 antibody [TRP/816] used in Protein Array (Protein Array). GTX34934
Anti-p53 antibody [TRP/816] used in IHC (Paraffin sections) (IHC-P). GTX34934

Cat No. GTX34934

Host

Mouse

Clonality

Monoclonal

Clone Name

TRP/816

Isotype

IgG2a

Application

WB, IHC-P, Protein Array

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Specificity/Sensitivity

Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human full-length TP53 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCC7 , LFS1 , P53 , TP53 , TRP53 , tumor protein p53 , p53

Cellular Localization

Cytoplasm,Nucleus,PML body,Endoplasmic reticulum,Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 antibody [TRP/816] used in Western Blot (WB). GTX34934

GTX34934 WB Image

WB analysis of HeLa cell lysate using GTX34934 p53 antibody [TRP/816].

Anti-p53 antibody [TRP/816] used in Protein Array (Protein Array). GTX34934

GTX34934 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using p53 Mouse Monoclonal Antibody (TP53/816). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-p53 antibody [TRP/816] used in IHC (Paraffin sections) (IHC-P). GTX34934

GTX34934 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX34934 p53 antibody [TRP/816].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf