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p53 (phospho Ser15) antibody

Anti-p53 (phospho Ser15) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132995
Anti-p53 (phospho Ser15) antibody used in Western Blot (WB). GTX132995

Cat No. GTX132995

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
100 μl ($319),
25 μl ($169)

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , 293T (0.5 μM doxorubicin for 24 hr)




1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.


Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.


0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species



Carrier-protein conjugated synthetic peptide corresponding to residues around human p53 (phospho Ser15). The exact sequence is proprietary.


Purified by antigen-affinity chromatography.




For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


tumor protein p53,BCC7,BMFS5,LFS1,P53,TRP53

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum


This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]


Research Area

Anti-p53 (phospho Ser15) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132995

GTX132995 ICC/IF Image

p53 (phospho Ser15) antibody detects p53 (phospho Ser15) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 (phospho Ser15) protein stained by p53 (phospho Ser15) antibody (GTX132995) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-p53 (phospho Ser15) antibody used in Western Blot (WB). GTX132995

GTX132995 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser15) antibody (GTX132995) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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p53 (phospho Ser15) antibody Cat No. GTX132995

Rating ( Average 5 based on 1 users reviews)
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 01-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40 μg
Blocking : 0.5% BSA, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Package List Price ($)
$ 319
$ 169