*Optimal dilutions/concentrations should be determined by the researcher.
|1-2μg/ml for 30 min at RT
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.
Recognizes a protein of 57kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1.
10mM PBS, 0.05% BSA
0.05% Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant human p57Kip2 protein
Protein A/G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
BWCR , BWS , CDKN1C , KIP2 , WBS , cyclin dependent kinase inhibitor 1C , p57 , p57Kip2 , p57 Kip2
This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
GTX34939 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX34939 p57 Kip2 antibody [KP10].