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p63 antibody [GT1179]

KO/KD Orthogonal
Anti-p63 antibody [GT1179] used in Western Blot (WB). GTX633818
Anti-p63 antibody [GT1179] used in Western Blot (WB). GTX633818
Anti-p63 antibody [GT1179] used in Western Blot (WB). GTX633818
Anti-p63 antibody [GT1179] used in IHC (Paraffin sections) (IHC-P). GTX633818
Anti-p63 antibody [GT1179] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633818

Cat. No. GTX633818

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1179

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all p63 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p63. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888381

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p63 , AIS , B(p51A) , B(p51B) , EEC3 , KET , LMS , NBP , OFC8 , RHS , SHFM4 , TP53CP , TP53L , TP73L , p40 , p51 , p53CP , p63 , p73H , p73L

Cellular Localization

Nucleus

Background

This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. Many transcripts encoding different proteins have been reported but the biological validity and the full-length nature of these variants have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p63 antibody [GT1179] used in Western Blot (WB). GTX633818

GTX633818 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [GT1179] (GTX633818) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p63 antibody [GT1179] used in Western Blot (WB). GTX633818

GTX633818 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [GT1179] (GTX633818) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-p63 antibody [GT1179] used in Western Blot (WB). GTX633818

GTX633818 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [GT1179] (GTX633818) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p63 antibody [GT1179] used in IHC (Paraffin sections) (IHC-P). GTX633818

GTX633818 IHC-P Image

p63 antibody [GT1179] detects p63 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
p63 stained by p63 antibody [GT1179] (GTX633818) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p63 antibody [GT1179] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633818

GTX633818 ICC/IF Image

p63 antibody [GT1179] detects p63 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p63 stained by p63 antibody [GT1179] (GTX633818) diluted at 1:200.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Mendoza-Rodr?guez MG et al. Int J Mol Sci 2019; 20 (2)
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Mendoza-Rodr?guez MG et al. Int J Mol Sci 2019; 20 (2)
Application : WB
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations