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p63 antibody [GT1179]

Anti-p63 antibody [GT1179] used in Western Blot (WB). GTX633818
Anti-p63 antibody [GT1179] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633818
Anti-p63 antibody [GT1179] used in IHC (Paraffin sections) (IHC-P). GTX633818

Cat No. GTX633818

Host Mouse
Clonality Monoclonal
Clone Name GT1179
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

A431 , TP63 shRNA-transfected A431

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p63. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tumor protein p63 , AIS , B(p51A) , B(p51B) , EEC3 , KET , LMS , NBP , OFC8 , RHS , SHFM4 , TP53CP , TP53L , TP73L , p40 , p51 , p53CP , p63 , p73H , p73L

Cellular Localization

Nucleus

Background

This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. Many transcripts encoding different proteins have been reported but the biological validity and the full-length nature of these variants have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-p63 antibody [GT1179] used in Western Blot (WB). GTX633818

GTX633818 WB Image

Non-transfected (–) and transfected (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [GT1179] (GTX633818) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p63 antibody [GT1179] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633818

GTX633818 ICC/IF Image

p63 antibody [GT1179] detects p63 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p63 stained by p63 antibody [GT1179] (GTX633818) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-p63 antibody [GT1179] used in IHC (Paraffin sections) (IHC-P). GTX633818

GTX633818 IHC-P Image

p63 antibody [GT1179] detects p63 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
p63 stained by p63 antibody [GT1179] (GTX633818) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169