Non-transfected (–) and transfected (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [HL1027] (GTX635841) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [HL1027] (GTX635841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

p63 antibody [HL1027] detects p63 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tongue.
p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p63 antibody [HL1027] detects p63 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human placenta.
p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p63 antibody [HL1027] detects p63 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p63 antibody [HL1027] detects p63 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

p63 antibody [HL1027] detects p63 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [HL1027] (GTX635841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.