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p63 antibody [N2C1], Internal

Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425
Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425
Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425
Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425
Anti-p63 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX102425
Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425
Anti-p63 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102425
Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425

Cat No. GTX102425

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP, PLA
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

A431 , TP63 shRNA-transfected A431 , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p63. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tumor protein p63 , AIS , B(p51A) , B(p51B) , EEC3 , KET , LMS , NBP , OFC8 , RHS , SHFM4 , TP53CP , TP53L , TP73L , p40 , p51 , p53CP , p63 , p73H , p73L

Cellular Localization

Nucleus

Background

This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. Many transcripts encoding different proteins have been reported but the biological validity and the full-length nature of these variants have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425

GTX102425 IHC-P Image

Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.
Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4℃ and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425

GTX102425 WB Image

Non-transfected (–) and transfected (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [N2C1], Internal (GTX102425) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425

GTX102425 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [N2C1], Internal (GTX102425) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p63 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102425

GTX102425 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using p63(GTX102425) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-p63 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX102425

GTX102425 IP Image

Immunoprecipitation of p63 protein from A431 whole cell extracts using 5 μg of p63 antibody [N2C1], Internal (GTX102425).
Western blot analysis was performed using p63 antibody [N2C1], Internal (GTX102425).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425

GTX102425 WB Image

p63 antibody [N2C1], Internal detects TP63 protein by western blot analysis.
A. 50 µg rat brain lysate/extract
7.5% SDS-PAGE
p63 antibody [N2C1], Internal (GTX102425) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p63 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102425

GTX102425 ICC/IF Image

p63 antibody [N2C1], Internal detects p63 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p63 stained by p63 antibody [N2C1], Internal (GTX102425) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Anti-p63 antibody [N2C1], Internal used in Western Blot (WB). GTX102425

GTX102425 WB Image

p63 antibody [N2C1], Internal detects TP63 protein by western blot analysis.
A. 50 µg mouse brain lysate/extract
7.5% SDS-PAGE
p63 antibody [N2C1], Internal (GTX102425) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

p63 antibody [N2C1], Internal Cat No. GTX102425

Rating ( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 19-Mar-2018
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : vas deferens tissue
Blocking : 10% normal goat serum + 0.025% triton-X100 in PBS, 25°C, 0.5Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : 0.025%triton-X100 in PBS
Primary Antibody : 1:400, 4°C, 6Hr
Date : Anonymous submitted on 21-Nov-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human TAp63gamma transfected 293T cells
Amount used : 10μg
Blocking : 2% BSA, 3% non-fat milk in PBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 18-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : Flag-human p63 alpha transfected Panc-1 cells
Amount used : 30μg
Blocking : 0.5% milk in TBST, 4°C, 12Hr
Primary Antibody : 1:2000, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169