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pan Cytokeratin antibody [B311.1]

Cat. No. GTX28474

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 B311.1

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat, Bovine
Package
500 μl ($399)
See all pan Cytokeratin products

APPLICATION

Application Note

Western Blotting 1:100-1:400This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100. High temperature antigenic unmasking technique or enzymatic predigestion is recommended with this antibody when using paraffin-embedded tissue sections.

Positive Control

Skin, Colonic Carcinoma

Product Note

This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond to cytokeratins 4,5,6,8,10,13 and 18.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.

Purification

Protein A affinity purified

Conjugation

Unconjugated

RRID

AB_385327

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

This antibody is a broad spectrum antibody and reacts with several normal and neoplastic epithelia. This antibody reacts with simple epithelium and both suprabasal and basal layers of cornifying and noncornifying squamous epithelium. It also works in smears of cultured epithelial cell lines.

Research Area

REFERENCE

Application Reference

Ordonez NG et al. Hum Pathol 2013; 44 (7) : 1195-215 Broad-spectrum immunohistochemical epithelial markers: a review.
Application : IHC-P
Forster S et al. Mod Pathol 2011; 24 (10) : 1390-403 THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling.
Application : IHC-Fr
Reactivity : Human
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399