Western Blotting 1:100-1:400This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100. High temperature antigenic unmasking technique or enzymatic predigestion is recommended with this antibody when using paraffin-embedded tissue sections.
Skin, Colonic Carcinoma
This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond to cytokeratins 4,5,6,8,10,13 and 18.
Phosphate-buffered saline, pH 7.2, containing 1% BSA and 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
0.4 mg/ml (Please refer to the vial label for the specific concentration.)
Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.
Protein A affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.