Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cytokeratin antibody [HL2253] (GTX638302) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cytokeratin antibody [HL2253] (GTX638302) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

pan Cytokeratin antibody [HL2253] detects pan Cytokeratin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
pan Cytokeratin stained by pan Cytokeratin antibody [HL2253] (GTX638302) diluted at 1:51.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min