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pro-Neuropeptide Y antibody

Anti-pro-Neuropeptide Y antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80749
Anti-pro-Neuropeptide Y antibody used in  (). GTX80749

Cat No. GTX80749

Host Guinea pig
Clonality Polyclonal
Application ICC/IF, IHC
Reactivity Mouse, Rat
Package
50 μl ($289)
APPLICATION

Application Note

Immunohistochemistry 1:500 (Dilutions listed only as a recommendation. Optimal dilution should be determined by investigator.) Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS.Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4oC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.

Calculated MW

11 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Whole Serum

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

SDLLMRESTENAPRTR Corresponding to residues 76– 91 of rat preproneuropeptide Y

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-pro-Neuropeptide Y antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80749

GTX80749 ICC/IF Image

NPY staining of striatal rat interneurones (arrows).

Anti-pro-Neuropeptide Y antibody used in  (). GTX80749

GTX80749 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

REFERENCE
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Package List Price ($)
$ 289