Detection of human recombinant proBNP expressed mammalian cells in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Antigen: recombinant proBNP expressed in mammalian cells, 0.15 ug; Lane 1: proBNP antibody [5B6] (GTX13111); Lane 2: proBNP antibody [15F11] (GTX13115); Lane 3: proBNP antibody [3G12] (GTX14712); Lane 4: proBNP antibody [11D1] (GTX13059); Lane 5: proBNP antibody [24E11] (GTX13123); Both proBNP antibody [11D1] (GTX13059) and proBNP antibody [24E11] (GTX13123)do not recognize glycosylated proBNP expressed in mammalian cells.