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proTUNEL DNA Fragmentation Assay Kit

proTUNEL DNA Fragmentation Assay Kit. GTX85585

Cat No. GTX85585


60 assay ($749)


Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. GeneTex’s proTUNEL In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.

‧ Detection method- Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters)
‧ Sample type- Cell culture (adherent and suspension), parafin embedded tissue sections
‧ Species reactivity- Mammalian
‧ Kit size- 60 assays
‧ Applications- The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.

Features and Benefits
‧ Simple procedure; takes ~ 1 hour
‧ Fast and convenient
‧ The assay is sensitive and stable
‧ The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods.


Content Volume Cap Color Storage
Positive Control Cells 5 ml brown -20 ºC
Negative Control Cells 5 ml natural -20 ºC
Wash Buffer 120 ml blue 4 ºC
Reaction Buffer 600 μl green 4 ºC
TdT Enzymes 45 μl yellow -20 ºC
Br-dUTP 480 μl violet -20 ºC
Rinse Buffer 120 ml red 4 ºC
Anti-BrdU-FITC Antibody 300 μl orange 4 ºC
PI/RNase Staining Buffer 30 ml amber bottle 4 ºC


Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.



Store at -20ºC. Product has an expected shelf life of 12 months.


For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.



TUNEL Assay , Apoptosis Assay , Terminal deoxynucleotidyl transferase dUTP nick end labeling assay , Apoptotic DNA Fragmentation Assay


proTUNEL DNA Fragmentation Assay Kit. GTX85585

GTX85585 Image



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Package List Price ($)
$ 749