Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. GeneTex’s proTUNEL In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.
‧ Detection method- Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters)
‧ Sample type- Cell culture (adherent and suspension), parafin embedded tissue sections
‧ Species reactivity- Mammalian
‧ Kit size- 60 assays
‧ Applications- The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Features and Benefits
‧ Simple procedure; takes ~ 1 hour
‧ Fast and convenient
‧ The assay is sensitive and stable
‧ The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods.