GeneTex
  • Country / Location Selection

United States (US)

proTUNEL DNA Fragmentation Assay Kit

proTUNEL DNA Fragmentation Assay Kit. GTX85585

Cat No. GTX85585

Application ICC/IF, FACS, IHC
Package
60 assay ($659)
PRODUCT

Product Description

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. GeneTex’s proTUNEL In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.

‧ Detection method- Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters)
‧ Sample type- Cell culture (adherent and suspension), parafin embedded tissue sections
‧ Species reactivity- Mammalian
‧ Kit size- 60 assays
‧ Applications- The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.

Features and Benefits
‧ Simple procedure; takes ~ 1 hour
‧ Fast and convenient
‧ The assay is sensitive and stable
‧ The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods.

Kit Contents:
Positive Control Cells
Negative Control Cells
Wash Buffer
Reaction Buffer
TdT Enzymes
Br-dUTP
Rinse Buffer
Anti-BrdU-FITC Antibody
PI/RNase Staining Buffer

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.
PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TUNEL Assay, Apoptosis Assay, Terminal deoxynucleotidyl transferase dUTP nick end labeling assay, Apoptotic DNA Fragmentation Assay
DATA IMAGES
proTUNEL DNA Fragmentation Assay Kit. GTX85585

GTX85585 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

REFERENCE
REVIEW
There are currently no reviews for proTUNEL DNA Fragmentation Assay Kit (GTX85585). Be the first to share your experience with this product.
Package List Price ($)
$ 659