GeneTex
United States (US)

sRANKL antibody

Anti-sRANKL antibody used in IHC (Paraffin sections) (IHC-P). GTX31300
Anti-sRANKL antibody used in Western Blot (WB). GTX31300

Cat No. GTX31300

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Rat Liver Tissue Lysate(GTX27943) , Human Liver Tissue Slide , Human Liver Tissue Lysate
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

sRANK-L antibody was raised against a 14 amino acid synthetic peptide from near the center of human sRANK-L.The immunogen is located within amino acids 140 - 190 of sRANK Ligand.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tnf Superfamily Member 11,Cd254,Odf,Opgl,Optb2,Rankl,Tnlg6B,Trance,Hrankl2,Sodf,Tnfsf11

Background

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced tra

Database

Research Area

DATA IMAGES
Anti-sRANKL antibody used in IHC (Paraffin sections) (IHC-P). GTX31300

GTX31300 IHC-P Image

IHC-P analysis of human liver tissue using GTX31300 sRANKL antibody.
Working concentration : 5 μg/ml

Anti-sRANKL antibody used in Western Blot (WB). GTX31300

GTX31300 WB Image

WB analysis of rat liver tissue lysate using GTX31300 sRANKL antibody.
Working concentration : (A) 0.25 and (B) 0.5 μg/ml

Package List Price ($)
$ 329