Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with tPA antibody (GTX103453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

tPA antibody detects PLAT protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
tPA antibody (GTX103453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

tPA antibody detects tPA protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
tPA antibody (GTX103453) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX103453 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

tPA antibody detects PLAT protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
tPA antibody (GTX103453) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with tPA antibody (GTX103453) diluted at 1:2000.

tPA antibody detects PLAT protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
7.5% SDS-PAGE
tPA antibody (GTX103453) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.