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RbAp48 antibody [13D10]

Orthogonal
Anti-RbAp48 antibody [13D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70234
Anti-RbAp48 antibody [13D10] used in Western Blot (WB). GTX70234
Anti-RbAp48 antibody [13D10] used in Immunoprecipitation (IP). GTX70234
Anti-RbAp48 antibody [13D10] used in Western Blot (WB). GTX70234

Cat. No. GTX70234

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 13D10

Isotype

 IgG2b

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human
Package
100 μl ($399)
See all RbAp48 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa nuclear extract , SH-SY-5Y , SH-SY-5Y nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_372871

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB binding protein 4, chromatin remodeling factor , NURF55 , RBAP48 , lin-53

Cellular Localization

Nucleus

Background

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES

Anti-RbAp48 antibody [13D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70234

GTX70234 ICC/IF Image

RbAp48 antibody [13D10] detects RbAp48 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RbAp48 protein stained by RbAp48 antibody [13D10] (GTX70234) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-RbAp48 antibody [13D10] used in Western Blot (WB). GTX70234

GTX70234 WB Image

Detection of RbAp48 in HeLa nuclear extract (GTX24105)

Anti-RbAp48 antibody [13D10] used in Immunoprecipitation (IP). GTX70234

GTX70234 IP Image

Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 μg of RbAp48 antibody [13D10] (GTX70234).
Western blot analysis was performed using RbAp48 antibody [13D10] (GTX70234).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RbAp48 antibody [13D10] used in Western Blot (WB). GTX70234

GTX70234 WB Image

Orthogonal

SH-SY5Y whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [13D10] (GTX70234) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kim MJ et al. Mol Cell 2021; 81 (8) : 1698-1714.e6 PAF remodels the DREAM complex to bypass cell quiescence and promote lung tumorigenesis.
Application : WB, IP
Reactivity : Human
Sun JM et al. J Biol Chem 2007; 282 (45) : 33227-36 Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin.
Application : WB
Reactivity : Human
Schultz DC et al. Genes Dev 2001; 15 (4) : 428-43 Targeting histone deacetylase complexes via KRAB-zinc finger proteins: the PHD and bromodomains of KAP-1 form a cooperative unit that recruits a novel isoform of the Mi-2alpha subunit of NuRD.
Application : WB
Reactivity : Human
Skowyra D et al. J Biol Chem 2001; 276 (12) : 2496312 Differential association of products of alternative transcripts of the candidate tumor suppressor ING1 with the mSin3/HDAC1 transcriptional corepressor complex.
Application : WB
Reactivity : Human
Humphrey GW et al. J Biol Chem 2001; 276 (9) : 6817-24 Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations