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SRC1 antibody [1135]

Anti-SRC1 antibody [1135] used in Western Blot (WB). GTX70250

Cat. No. GTX70250

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1135

Isotype

 IgG1

Application

 WB, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all SRC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 0.5 - 2 μg/ml
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

157 kDa. ( Note )

Positive Control

mouse brain

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 477-947 of human SRC-1.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_373065

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor coactivator 1 , F-SRC-1 , KAT13A , RIP160 , SRC1 , bHLHe42 , bHLHe74

Cellular Localization

Nucleus

Background

The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SRC1 antibody [1135] used in Western Blot (WB). GTX70250

GTX70250 WB Image

Mouse tissue (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] (GTX70250) diluted at 1:2000.

REFERENCE

Application Reference

Amita M et al. Endocrinology 2010; 151 (1) : 394-405 molecular mechanism of the inhibition of *-induced endometrial epithelial cell proliferation by * citrate.
Application : WB
Reactivity : Human
Jaber BM et al. Mol Endocrinol 2006; 20 (11) : 2695-710 The pure estrogen receptor antagonist ICI 182,780 promotes a novel interaction of estrogen receptor-alpha with the 3',5'-cyclic adenosine monophosphate response element-binding protein-binding protein/p300 coactivators.
Application : IP
Reactivity : Human
Klinge CM et al. J Mol Endocrinol 2004; 33 (2) : 387-410 Estrogen response element-dependent regulation of transcriptional activation of estrogen receptors alpha and beta by coactivators and corepressors.
Application : WB
Reactivity : Human
Dutertre M et al. Mol Endocrinol 2003; 17 (7) : 1296-314 Ligand-independent interactions of p160/steroid receptor coactivators and CREB-binding protein (CBP) with estrogen receptor-alpha: regulation by phosphorylation sites in the A/B region depends on other receptor domains.
Application : IP
Reactivity : Human
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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations