EIF2B epsilon (phospho Ser539) antibody

Name: EIF2B epsilon (phospho Ser539) antibody
Brand: GeneTex
SKU: GTX24775
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-EIF2B epsilon (phospho Ser539) antibody used in Western Blot (WB). GTX24775

Cat. No. GTX24775

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse, Rat
Package	50 μl ($399)

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View product citations for antibody GTX24775 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent

Not tested in other applications.

Calculated MW

80 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat eIF2Be that contains serine 535 (corresponding to serine 539 in the human sequence).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380420

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2B subunit epsilon , CACH , CLE , EIF-2B , EIF2Bepsilon , LVWM

Background

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Nov 2009]

Database

Gene ID: 8893 EIF2B5
UniProt: Q13144 EIF2B5

Research Area

DATA IMAGES

GTX24775 WB Image

WB (peptide competition) analysis of recombinant eIF2B epsilon treated with lambda phosphatase (Lane 1) or GSK-3beta (Lane 2-5) using GTX24775 EIF2B epsilon (phospho Ser539) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

Application Reference

Tardif N et al. Aging Cell 2014; Muscle ectopic fat deposition contributes to anabolic resistance in obese sarcopenic old rats through eIF2α activation.

Application : WB

Reactivity : Mouse

Mascher H et al. Acta Physiol (Oxf) 2011; 202 (2) : 175-84 Enhanced rates of muscle protein synthesis and elevated mTOR signalling following endurance exercise in human subjects.

Burd NA et al. J Physiol 2010; 588 (Pt 16) : 3119-30 Resistance exercise volume affects myofibrillar protein synthesis and anabolic signalling molecule phosphorylation in young men.

Application : WB

Reactivity : Human

Fry CS et al. J Appl Physiol (1985) 2010; 108 (5) : 1199-209 Blood flow restriction exercise stimulates mTORC1 signaling and muscle protein synthesis in older men.

Application : WB

Reactivity : Human

Moore DR et al. Am J Clin Nutr 2009; 89 (1) : 161-8 Ingested protein dose response of muscle and albumin protein synthesis after resistance exercise in young men.

Application : WB

Reactivity : Human

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