CEP55 antibody [N2C3]

Name: CEP55 antibody [N2C3]
Brand: GeneTex
SKU: GTX112190
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-CEP55 antibody [N2C3] used in Western Blot (WB). GTX112190

Cat. No. GTX112190

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX112190 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CEP55. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617544

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centrosomal protein 55 , C10orf3 , CT111 , MARCH , URCC6

Cellular Localization

Centrosome , centriole

Background

Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.

Database

Gene ID: 55165 CEP55
UniProt: Q53EZ4 CEP55

Research Area

DATA IMAGES

GTX112190 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX112190 diluted at 1:1000

REFERENCE

Application Reference

Petsalaki E et al. J Cell Biol 2023; 222 (11) The abscission checkpoint senses chromatin bridges through Top2α recruitment to DNA knots.

Eleni Petsalaki et al. J Cell Biol 2021; 220 (2) An ATM-Chk2-INCENP pathway activates the abscission checkpoint.

Application : WB, ICC/IF

Reactivity : Human

Kuo TC et al. Nat Cell Biol 2011; 13 (10) : 1214-23 Midbody accumulation through evasion of autophagy contributes to cellular reprogramming and tumorigenicity.

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