GeneTex

  • Country / Location Selection

United States (US)

ERR alpha antibody [N1], N-term

Orthogonal
Anti-ERR alpha antibody [N1], N-term used in Immunoprecipitation (IP). GTX108166
Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166
Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166
Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166
Anti-ERR alpha antibody [N1], N-term used in ChIP assay (ChIP assay). GTX108166
Anti-ERR alpha antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108166
Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166
Anti-ERR alpha antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108166
Anti-ERR alpha antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108166
Anti-ERR alpha antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108166
Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166
Anti-ERR alpha antibody [N1], N-term used in Western Blot and Immunoprecipitation (WB IP). GTX108166
Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166

Cat. No. GTX108166

Reference 14
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ERR alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

293T , A431 , H1299

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human ERR alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950230

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

estrogen related receptor alpha , ERR1 , ERRa , ERRalpha , ESRL1 , NR3B1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ERR alpha antibody [N1], N-term used in Immunoprecipitation (IP). GTX108166

GTX108166 IP Image

ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody (GTX108166) 7.5% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody (GTX108166) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166

GTX108166 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166

GTX108166 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
12% SDS PAGE
GTX108166 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166

GTX108166 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERR alpha antibody [N1], N-term used in ChIP assay (ChIP assay). GTX108166

GTX108166 ChIP assay Image

Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.

Anti-ERR alpha antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108166

GTX108166 IHC-P Image

ERR alpha antibody [N1], N-term detects ERR alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ERR alpha stained by ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166

GTX108166 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERR alpha antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108166

GTX108166 IHC-P Image

ERR alpha antibody [N1], N-term detects ERR alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ERR alpha stained by ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ERR alpha antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108166

GTX108166 IHC-P Image

ERR alpha antibody [N1], N-term detects ERR alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
ERR alpha stained by ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ERR alpha antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108166

GTX108166 ICC/IF Image

ERR alpha antibody [N1], N-term detects ERR alpha protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERR alpha stained by ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166

GTX108166 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 25781607

Anti-ERR alpha antibody [N1], N-term used in Western Blot and Immunoprecipitation (WB IP). GTX108166

GTX108166 WB IP Image

Orthogonal

The data was published in the journal PLoS One in 2017. PMID: 29190800

Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166

GTX108166 WB Image

The data was published in the journal PLoS One in 2017. PMID: 29190800

REFERENCE

Application Reference

Xu DM et al. Heliyon 2024; 10 (1) : e23739 ERRα regulates synaptic transmission through reactive oxygen species in hippocampal neurons.
DM Xu et al. Heliyon 2024; 10 (1) : e23739 ERRα regulates synaptic transmission through reactive oxygen species in hippocampal neurons
Application : WB
Nowi?ska K et al. Int J Mol Sci 2022; 23 (22) Association of Irisin/FNDC5 with ERRα and PGC-1α Expression in NSCLC.
Panpan Dong et al. Exp Ther Med 2022; 23 (2) : 164 Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 ?M bisphenol A exposure via human neural stem cells.
Application : ChIP assay
Reactivity : Human
Cerutti C et al. Sci Rep 2022; 12 (1) : 3826 Computational identification of new potential transcriptional partners of ERRα in breast cancer cells: specific partners for specific targets.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

ERR alpha antibody [N1], N-term Cat. No. GTX108166

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166
Date : Anonymous submitted on 28-Mar-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40μg
Blocking : 5% milk, 23°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Submit a Review
Application Reference
Xu DM et al. Heliyon 2024; 10 (1):e23739 ERRα regulates synaptic transmission through reactive oxygen species in hippocampal neurons.
DM Xu et al. Heliyon 2024; 10 (1):e23739 ERRα regulates synaptic transmission through reactive oxygen species in hippocampal neurons
Application : WB
Nowi?ska K et al. Int J Mol Sci 2022; 23 (22) Association of Irisin/FNDC5 with ERRα and PGC-1α Expression in NSCLC.
Panpan Dong et al. Exp Ther Med 2022; 23 (2):164 Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 ?M bisphenol A exposure via human neural stem cells.
Application : ChIP assay
Reactivity : Human
Cerutti C et al. Sci Rep 2022; 12 (1):3826 Computational identification of new potential transcriptional partners of ERRα in breast cancer cells: specific partners for specific targets.
Application : WB
Reactivity : Human
Tsushida K et al. Biochem Biophys Res Commun 2018; (Epub) estrogen-related receptor α is essential for maintaining mitochondrial integrity in *-induced acute kidney injury.
Application : WB
Reactivity : Mouse
Carnesecchi J et al. PLoS One 2017; 12 (11):e0188871 ERRα protein is stabilized by LSD1 in a demethylation-independent manner.
Application : WB
Reactivity : Human
Puigserver P et al. Mol Cancer Res 2017; (Epub) ERRα maintains Mitochondrial Oxidative Metabolism and Constitutes an Actionable Target in PGC1α-elevated Melanomas.
Application : WB
Reactivity : Human
Carnesecchi J et al. Proc Natl Acad Sci U S A 2017; 114 (15):3909-3914 ERRα induces H3K9 demethylation by LSD1 to promote cell invasion.
Application : WB, ChIP assay
Reactivity : Human
Tribollet V et al. PLoS One 2016; 11 (5):e0156445 miR-135a Inhibits the Invasion of Cancer Cells via Suppression of ERRα.
Application : WB
Reactivity : Human
Carnesecchi J et al. PLoS One 2015; 10 (3):e0120672 Estrogens induce rapid cytoskeleton re-organization in human dermal fibroblasts via the non-classical receptor GPR30.
Application : WB
Reactivity : Human
Sailland J et al. Proc Natl Acad Sci U S A 2014; 111 (42):15108-13 Estrogen-related receptor α decreases RHOA stability to induce orientated cell migration.
Kammerer M et al. Mol Cell Endocrinol 2013; 369 (43102):150-60 Estrogen Receptor α (ERα) and Estrogen Related Receptor α (ERRα) are both transcriptional regulators of the Runx2-I isoform.
Application : WB
Cai Q et al. Oncogene 2013; 32 (16):2079-86 Regulation of glycolysis and the Warburg effect by estrogen-related receptors.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169