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PDI antibody [N1N3]

Anti-PDI antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101468
Anti-PDI antibody [N1N3] used in Western Blot (WB). GTX101468
Anti-PDI antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101468
Anti-PDI antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101468
Anti-PDI antibody [N1N3] used in Western Blot (WB). GTX101468
Anti-PDI antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101468

Cat. No. GTX101468

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, EM

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PDI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
EM Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

Mouse liver , 293T , A431 , H1299

Predict Reactivity

Rabbit, Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241147

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prolyl 4-hydroxylase subunit beta , CLCRP1 , DSI , ERBA2L , GIT , P4Hbeta , PDI , PDIA1 , PHDB , PO4DB , PO4HB , PROHB

Cellular Localization

Endoplasmic reticulum lumen , Melanosome , Cell membrane; Peripheral membrane protein (Potential)

Background

This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PDI antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101468

GTX101468 ICC/IF Image

PDI antibody [N1N3] detects PDI protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDI protein stained by PDI antibody [N1N3] (GTX101468) diluted at 1:100.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-PDI antibody [N1N3] used in Western Blot (WB). GTX101468

GTX101468 WB Image

Sample (20 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX101468 diluted at 1:10000

Anti-PDI antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101468

GTX101468 IHC-P Image

PDI antibody [N1N3] detects PDI protein at cytosol and membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PDI antibody [N1N3] (GTX101468) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PDI antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101468

GTX101468 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H520 xenograft, using PDI(GTX101468) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PDI antibody [N1N3] used in Western Blot (WB). GTX101468

GTX101468 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
7.5% SDS PAGE
GTX101468 diluted at 1:500

Anti-PDI antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101468

GTX101468 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using PDI(GTX101468) antibody at 1:200 dilution.

REFERENCE

Application Reference

Leu JG et al. Life (Basel) 2021; 11 (12) The Cell Protective Effect of Adenine on Hypoxia-Reoxygenation Injury through PPAR Delta Activation.
Application : WB
Reactivity : Rat
Lee LC et al. J Neural Transm 2014; Downregulation of proteins involved in the endoplasmic reticulum stress response and Nrf2-ARE signaling in lymphoblastoid cells of spinocerebellar ataxia type 17.
Application : WB
Reactivity : Human
Chen JY et al. J Nutr Biochem 2013; 24 (11) : 1889-910 High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Application : WB
Reactivity : Human
Giordano F et al. Cell 2013; 153 (7) : 1494-509 PI(4,5)P(2)-dependent and Ca(2+)-regulated ER-PM interactions mediated by the extended synaptotagmins.
Application : EM
Reactivity : Human
Shi-Chen Ou D et al. Cell Res 2011; 21 (4) : 642-53 Transcriptional activation of endoplasmic reticulum chaperone GRP78 by HCMV IE1-72 protein.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169