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PKC zeta antibody

Anti-PKC zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX111598
Anti-PKC zeta antibody used in Western Blot (WB). GTX111598
Anti-PKC zeta antibody used in Western Blot (WB). GTX111598
Anti-PKC zeta antibody used in Western Blot (WB). GTX111598

Cat. No. GTX111598

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all PKC zeta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKC zeta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951434

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C zeta , PKC-ZETA , PKC2

Cellular Localization

Cytoplasm , Endosome

Background

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PKC zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX111598

GTX111598 IHC-P Image

PKC zeta antibody detects PKC zeta protein at cytoplasm on SW480 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SW480 xenograft.
PKC zeta antibody (GTX111598) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PKC zeta antibody used in Western Blot (WB). GTX111598

GTX111598 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC zeta antibody (GTX111598) diluted at 1:1000.

Anti-PKC zeta antibody used in Western Blot (WB). GTX111598

GTX111598 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish brain
7.5% SDS PAGE
GTX111598 diluted at 1:500

Anti-PKC zeta antibody used in Western Blot (WB). GTX111598

GTX111598 WB Image

The data was published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23840253

REFERENCE

Application Reference

Lee SY et al. Evid Based Complement Alternat Med 2013; 2013 : 284150 Rhodiola crenulata and Its Bioactive Components, Salidroside and Tyrosol, Reverse the Hypoxia-Induced Reduction of Plasma-Membrane-Associated Na,K-ATPase Expression via Inhibition of ROS-AMPK-PKC ξ Pathway.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169