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RACGAP1 antibody [N1N2], N-term

Anti-RACGAP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113320
Anti-RACGAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113320
Anti-RACGAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113320
Anti-RACGAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX113320
Anti-RACGAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX113320

Cat. No. GTX113320

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RACGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

Mouse heart , A431 , Raji

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RACGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951590

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rac GTPase activating protein 1 , CYK4 , HsCYK-4 , ID-GAP , MgcRacGAP

Cellular Localization

Nucleus , Cytoplasm , Spindle , Acrosome

Background

Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RACGAP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113320

GTX113320 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using RACGAP1(GTX113320) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-RACGAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113320

GTX113320 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using RACGAP1(GTX113320) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RACGAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113320

GTX113320 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using RACGAP1(GTX113320) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RACGAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX113320

GTX113320 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
7.5% SDS PAGE
GTX113320 diluted at 1:1000

Anti-RACGAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX113320

GTX113320 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Raji
7.5% SDS PAGE
GTX113320 diluted at 1:1000

REFERENCE

Application Reference

Chang YC et al. J Agric Food Chem 2013; 61 (49) : 12044-52 Interruption of Lung Cancer Cell Migration and Proliferation by Fungal Immunomodulatory Protein FIP-fve from Flammulina velutipes.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169