GeneTex

  • Country / Location Selection

United States (US)

TSG101 antibody

KO/KD
Anti-TSG101 antibody used in Western Blot (WB). GTX118736
Anti-TSG101 antibody used in IHC (Paraffin sections) (IHC-P). GTX118736
Anti-TSG101 antibody used in ELISA (ELISA). GTX118736
Anti-TSG101 antibody used in Western Blot (WB). GTX118736
Anti-TSG101 antibody used in Western Blot (WB). GTX118736
Anti-TSG101 antibody used in Immunoprecipitation (IP). GTX118736
Anti-TSG101 antibody used in IHC (Paraffin sections) (IHC-P). GTX118736
Anti-TSG101 antibody used in Western Blot (WB). GTX118736
Anti-TSG101 antibody used in Western Blot (WB). GTX118736
Anti-TSG101 antibody used in Western Blot (WB). GTX118736

Cat. No. GTX118736

Reference 25

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TSG101 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX118736 , Detection: GTX70255
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TSG101. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619853

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor susceptibility 101 , TSG10 , VPS23

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Late endosome membrane; Peripheral membrane protein

Background

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TSG101 antibody used in Western Blot (WB). GTX118736

GTX118736 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TSG101 antibody used in IHC (Paraffin sections) (IHC-P). GTX118736

GTX118736 IHC-P Image

TSG101 antibody detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
TSG101 stained by TSG101 antibody (GTX118736) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TSG101 antibody used in ELISA (ELISA). GTX118736

GTX118736 ELISA Image

ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 μg/mL and GTX70255 for detection at a concentration of 1.5 μg/mL.

Anti-TSG101 antibody used in Western Blot (WB). GTX118736

GTX118736 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TSG101 antibody used in Western Blot (WB). GTX118736

GTX118736 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TSG101 antibody used in Immunoprecipitation (IP). GTX118736

GTX118736 IP Image

Immunoprecipitation of TSG101 protein from A549 whole cell extracts using 5 μg of TSG101 antibody (GTX118736).
Western blot analysis was performed using TSG101 antibody (GTX118736) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TSG101 antibody used in IHC (Paraffin sections) (IHC-P). GTX118736

GTX118736 IHC-P Image

TSG101 antibody detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
TSG101 stained by TSG101 antibody (GTX118736) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TSG101 antibody used in Western Blot (WB). GTX118736

GTX118736 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TSG101 antibody used in Western Blot (WB). GTX118736

GTX118736 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TSG101 antibody used in Western Blot (WB). GTX118736

GTX118736 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Nguyen HT et al. Front Oncol 2024; 14 : 1346312 Extracellular vesicles derived from SARS-CoV-2 M-protein-induced triple negative breast cancer cells promoted the ability of tissue stem cells supporting cancer progression.
Hong CT et al. Elife 2024; 12 Plasma extracellular vesicle synaptic proteins as biomarkers of clinical progression in patients with Parkinson's disease.
Cheng CA et al. Adv Sci (Weinh) 2024; 11 (3) : e2304926 Ultrasensitive and High-Resolution Protein Spatially Decoding Framework for Tumor Extracellular Vesicles.
Hsu XR et al. J Exp Clin Cancer Res 2023; 42 (1) : 283 Exosomal long noncoding RNA MLETA1 promotes tumor progression and metastasis by regulating the miR-186-5p/EGFR and miR-497-5p/IGF1R axes in non-small cell lung cancer.
Chan L et al. In Vivo 2023; 37 (5) : 2028-2038 Therapeutic Effect of Human Adipocyte-derived Stem Cell-derived Exosomes on a Transgenic Mouse Model of Parkinson's Disease.
Show More +
Submit a Reference

REVIEW

There are currently no reviews for TSG101 antibody (GTX118736). Be the first to share your experience with this product.
Submit a Review
Application Reference
Nguyen HT et al. Front Oncol 2024; 14:1346312 Extracellular vesicles derived from SARS-CoV-2 M-protein-induced triple negative breast cancer cells promoted the ability of tissue stem cells supporting cancer progression.
Hong CT et al. Elife 2024; 12 Plasma extracellular vesicle synaptic proteins as biomarkers of clinical progression in patients with Parkinson's disease.
Cheng CA et al. Adv Sci (Weinh) 2024; 11 (3):e2304926 Ultrasensitive and High-Resolution Protein Spatially Decoding Framework for Tumor Extracellular Vesicles.
Hsu XR et al. J Exp Clin Cancer Res 2023; 42 (1):283 Exosomal long noncoding RNA MLETA1 promotes tumor progression and metastasis by regulating the miR-186-5p/EGFR and miR-497-5p/IGF1R axes in non-small cell lung cancer.
Chan L et al. In Vivo 2023; 37 (5):2028-2038 Therapeutic Effect of Human Adipocyte-derived Stem Cell-derived Exosomes on a Transgenic Mouse Model of Parkinson's Disease.
Benayas B et al. Front Bioeng Biotechnol 2023; 11:1238898 Proof of concept of using a membrane-sensing peptide for sEVs affinity-based isolation.
Beatriz Benayas et al. J Ex Bio 2023; 2:e100 Optimization of extracellular vesicle isolation and their separation from lipoproteins by size exclusion chromatography
Application : WB
Lin Z et al. Int J Mol Sci 2023; 24 (24) Therapeutic Potential of Adipose-Derived Stem Cell-Conditioned Medium and Extracellular Vesicles in an In Vitro Radiation-Induced Skin Injury Model.
Lim K et al. J Extracell Vesicles 2021; 10 (14):e12170 Millisecond dynamic of SARS-CoV-2 spike and its interaction with ACE2 receptor and small extracellular vesicles.
Application : WB
Reactivity : Human
Clerici SP et al. Front Cell Dev Biol 2021; 9:696373 Colorectal Cancer Cell-Derived Small Extracellular Vesicles Educate Human Fibroblasts to Stimulate Migratory Capacity.
Application : WB
Reactivity : Human
Chen-Chih Chung et al. Ther Adv Neurol Disord 2020; 13:1.75629E+15 Neurofilament light chain level in plasma extracellular vesicles and Parkinson's disease.
Application : WB
Reactivity : Human
Chung CC et al. Diagnostics (Basel) 2020; 10 (9) Plasma Exosomal Brain-Derived Neurotrophic Factor Correlated with the Postural Instability and Gait Disturbance-Related Motor Symptoms in Patients with Parkinson's Disease.
Application : WB
Reactivity : Human
Jean-Toussaint R et al. J Proteomics 2020; 211:103540 Proteome characterization of small extracellular vesicles from spared nerve injury model of neuropathic pain.
Application : WB
Reactivity : Mouse
Song HY et al. Cells 2019; 8 (4) Generation of GLA-Knockout Human Embryonic Stem Cell Lines to Model Autophagic Dysfunction and Exosome Secretion in Fabry Disease-Associated Hypertrophic Cardiomyopathy.
Application : WB
Reactivity : Human
Chua HH et al. Int J Mol Sci 2019; 20 (3) Cancer-Specifically Re-Spliced TSG101 mRNA Promotes Invasion and Metastasis of Nasopharyngeal Carcinoma.
Application : WB
Reactivity : Human
Park J et al. ACS Photonics 2018; 5 (2):487-494 Analyses of Intravesicular Exosomal Proteins Using a Nano-Plasmonic System.
Asada K et al. Sci Rep 2018; 8 (1):8551 Cytosolic Genomic DNA functions as a Natural Antisense.
Application : WB
Reactivity : Mouse
Lee HY et al. Oncotarget 2018; 9 (17):13193-13205 EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.
Application : WB
Reactivity : Human
Park J et al. ACS Photonics 2017; (Epub) Analyses of intravesicular exosomal proteins using a nano-plasmonic system.
Application : WB
Reactivity : Human
Lin MC et al. J Vis Exp 2017; (124) Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes.
Application : WB
Urata S et al. J Virol 2015; (Epub) Analysis of assembly and budding of Lujo virus.
Application : WB
Reactivity : Human
Taylor HE et al. J Virol 2014; 88 (1):592-603 The innate immune factor apolipoprotein L1 restricts HIV-1 infection.
Application : WB
Reactivity : Human
Edgar JR et al. Traffic 2014; 15 (2):197-211 Hrs- and CD63-dependent competing mechanisms make different sized endosomal intraluminal vesicles.
Application : WB
Reactivity : Human
Scotti E et al. Mol Cell Biol 2013; 33 (8):1503-14 IDOL stimulates clathrin-independent endocytosis and multivesicular body-mediated lysosomal degradation of the low-density lipoprotein receptor.
Application : WB
Reactivity : Human
Pasqual G et al. PLoS Pathog 2011; 7 (9):e1002232 Old world arenaviruses enter the host cell via the multivesicular body and depend on the endosomal sorting complex required for transport.
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169